7BW0

Active human TGR5 complex with a synthetic agonist 23H

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Soluble cytochrome b562,G-protein coupled bile acid receptor 1	polymer	427	46181.4	1	UniProt (P0ABE7) UniProt (Q8TDU6) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,G-protein coupled receptor GPCR19,hGPCR19,Membrane-type receptor for bile acids,M-BAR,hBG37,BG37
2	B (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	373	43385.2	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	357	39286.9	1	UniProt (P54311) Pfam (PF25391)	Rattus norvegicus (Rat)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
5	E (N)	Nanobody Nb35	polymer	157	17057.3	1		Vicugna pacos

Sequence modifications

R: -100 - 5 (UniProt: P0ABE7)

PDB	External Database	Details
Ala -119	-	expression tag
Asp -118	-	expression tag
Tyr -117	-	expression tag
Lys -116	-	expression tag
Asp -115	-	expression tag
Asp -114	-	expression tag
Asp -113	-	expression tag
Asp -112	-	expression tag
Lys -111	-	expression tag
Leu -110	-	expression tag
Glu -109	-	expression tag
Val -108	-	expression tag
Leu -107	-	expression tag
Phe -106	-	expression tag
Gln -105	-	expression tag
Gly -104	-	expression tag
Pro -103	-	expression tag
Gly -102	-	expression tag
Ser -101	-	expression tag
Trp -94	Met 29	conflict
Ile 1	His 124	conflict
Leu 5	Arg 128	conflict

R: 6 - 307 (UniProt: Q8TDU6)
A: 26 - 55 (UniProt: P63092)

PDB	External Database	Details
Met 8	-	initiating methionine
Gly 9	-	expression tag
Cys 10	-	expression tag
Thr 11	-	expression tag
Leu 12	-	expression tag
Ser 13	-	expression tag
Ala 14	-	expression tag
Glu 15	-	expression tag
Asp 16	-	expression tag
Lys 17	-	expression tag
Ala 18	-	expression tag
Ala 19	-	expression tag
Val 20	-	expression tag
Glu 21	-	expression tag
Arg 22	-	expression tag
Ser 23	-	expression tag
Lys 24	-	expression tag
Met 25	-	expression tag

A: 56 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	153772.0
All*	Total formula weight	153772.0

*Water molecules are not included.