7BVC

Cryo-EM structure of Mycobacterium smegmatis arabinosyltransferase EmbA-EmbB-AcpM2 in complex with ethambutol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Integral membrane indolylacetylinositol arabinosyltransferase EmbA	polymer	1088	116561.9	1	UniProt (A0R613) Pfam (PF17689) Pfam (PF04602) Pfam (PF14896) In PDB	Mycolicibacterium smegmatis MC2 155	Probable arabinosyltransferase B
2	B	Integral membrane indolylacetylinositol arabinosyltransferase EmbB	polymer	1100	119077.7	1	UniProt (I7GAQ2) Pfam (PF17689) Pfam (PF04602) Pfam (PF14896) UniProt (by SIFTS) (A0R614) In PDB	Mycolicibacterium smegmatis MC2 155
3	P	Meromycolate extension acyl carrier protein	polymer	99	10743.9	1	UniProt (A0R0B3) Pfam (PF00550) In PDB	Mycolicibacterium smegmatis MC2 155	ACP
4	A, B	CARDIOLIPIN	non-polymer		1464.0	2	Chemie (CDL)
5	A	[(2Z,6E,10E,14Z,18E,22Z,26Z)-3,7,11,15,19,23,27,31,35,39-decamethyltetraconta-2,6,10,14,18,22,26,30,34,38-decaenyl] [(2S,3S,4S,5R)-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl] hydrogen phosphate	non-polymer		911.3	1	Chemie (F8L)
6	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
7	B	4'-PHOSPHOPANTETHEINE	non-polymer		358.3	1	Chemie (PNS)
8	B	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
9	B	Ethambutol	non-polymer		204.3	1	Chemie (95E)

Sequence modifications

A: 1 - 1080 (UniProt: A0R613)

PDB	External Database	Details
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag

B: 1 - 1082 (UniProt: I7GAQ2)

PDB	External Database	Details
His 1083	-	expression tag
Leu 1084	-	expression tag
Gly 1085	-	expression tag
Gly 1086	-	expression tag
Ile 1087	-	expression tag
Lys 1088	-	expression tag
Ala 1089	-	expression tag
Phe 1090	-	expression tag
His 1091	-	expression tag
His 1092	-	expression tag
His 1093	-	expression tag
His 1094	-	expression tag
His 1095	-	expression tag
His 1096	-	expression tag
His 1097	-	expression tag
His 1098	-	expression tag
His 1099	-	expression tag
His 1100	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	246383.4
Non-Polymers*	Number of molecules	8
	Total formula weight	4577.2
All*	Total formula weight	250960.6

*Water molecules are not included.