7BHG

Gene-engineered variant of FusionRed with Trp based chromophore - 2.1A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Fluorescent protein FP480	polymer	249	28157.2	2	UniProt (D0VX33) Pfam (PF01353)	Entacmaea quadricolor (Bubble-tip anemone)
2	C, D (A, B)	water	water		18.0	142	Chemie (HOH)

Sequence modifications

A, B: 2 - 233 (UniProt: D0VX33)

PDB	External Database	Details
Met -17	-	initiating methionine
Arg -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Leu -5	-	expression tag
Pro -4	-	expression tag
Val -3	-	expression tag
Ala -2	-	expression tag
Thr -1	-	expression tag
Met 0	-	expression tag
Val 1	-	expression tag
Lys 6	Thr 6	conflict
Pro 10	His 10	conflict
Thr 11	Met 11	conflict
Met 41	Gln 41	conflict
Tuk 65	Met 63	chromophore
Tuk 65	Tyr 64	chromophore
Tuk 65	Gly 65	chromophore
Arg 67	His 67	conflict
Lys 71	Asn 71	conflict
Pro 73	Thr 73	conflict
Pro 74	Gln 74	conflict
Phe 80	Trp 80	conflict
Val 121	Ile 121	conflict
Ser 143	His 143	conflict
Thr 146	Met 146	conflict
Met 147	Leu 147	conflict
Cys 158	Ala 158	conflict
Met 160	Leu 160	conflict
Leu 174	Phe 174	conflict
Thr 185	Lys 185	conflict
Lys 186	Asn 186	conflict
His 197	Tyr 197	conflict
Asp 207	Lys 207	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56314.3
All*	Total formula weight	56314.3

*Water molecules are not included.