7BHG
Gene-engineered variant of FusionRed with Trp based chromophore - 2.1A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.846, 92.234, 57.997 |
Unit cell angles | 90.00, 111.83, 90.00 |
Refinement procedure
Resolution | 28.990 - 2.090 |
R-factor | 0.1666 |
Rwork | 0.163 |
R-free | 0.22970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | FusionRed |
RMSD bond length | 0.017 |
RMSD bond angle | 2.064 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
High resolution limit [Å] | 2.090 | 4.520 | 2.100 |
Rmerge | 0.066 | 0.046 | 0.362 |
Rmeas | 0.077 | 0.054 | 0.438 |
Rpim | 0.039 | 0.028 | 0.243 |
Number of reflections | 26662 | 2728 | 2543 |
<I/σ(I)> | 7.9 | ||
Completeness [%] | 99.6 | 100 | 96.1 |
Redundancy | 3.7 | 3.8 | 3 |
CC(1/2) | 0.997 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2mkl of protein solution 20 mM Tris pH 8.0 200mM NaCl mixed with 2mkl of 0.2M NaCl, 0.1M Bis-Tris 6.5, 25% PEG3350 |