7B6O

Crystal structure of E.coli MurE mutant - C269S C340S C450S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase	polymer	496	53445.3	2	UniProt (P22188) Pfam (PF01225) Pfam (PF08245) Pfam (PF02875)	Escherichia coli (strain K12)	Meso-A2pm-adding enzyme,Meso-diaminopimelate-adding enzyme,UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase,UDP-MurNAc-tripeptide synthetase,UDP-N-acetylmuramyl-tripeptide synthetase
2	C, D (A, B)	ISOPROPYL ALCOHOL	non-polymer		60.1	2	Chemie (IPA)
3	E, F (A, B)	water	water		18.0	318	Chemie (HOH)

Sequence modifications

A, B: 1 - 495 (UniProt: P22188)

PDB	External Database	Details
Ser 0	-	expression tag
Ser 269	Cys 269	engineered mutation
Ser 340	Cys 340	engineered mutation
Ser 450	Cys 450	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	106890.6
Non-Polymers*	Number of molecules	2
	Total formula weight	120.2
All*	Total formula weight	107010.8

*Water molecules are not included.