7B5N
Ubiquitin ligation to F-box protein substrates by SCF-RBR E3-E3 super-assembly: NEDD8-CUL1-RBX1-UBE2L3~Ub~ARIH1.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | Cullin-1 | polymer | 776 | 89800.4 | 1 | UniProt (Q13616) Pfam (PF00888) Pfam (PF10557) | Homo sapiens (Human) | CUL-1 |
| 2 | B (H) | E3 ubiquitin-protein ligase ARIH1 | polymer | 557 | 64197.8 | 1 | UniProt (Q9Y4X5) Pfam (PF21235) Pfam (PF00097) Pfam (PF01485) Pfam (PF22191) Pfam (PF19422) | Homo sapiens (Human) | H7-AP2,HHARI,Monocyte protein 6,MOP-6,Protein ariadne-1 homolog,ARI-1,UbcH7-binding protein,UbcM4-interacting protein,Ubiquitin-conjugating enzyme E2-binding protein 1 |
| 3 | C (U) | Polyubiquitin-C | polymer | 75 | 8519.8 | 1 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (Human) | |
| 4 | D (N) | NEDD8 | polymer | 81 | 9086.6 | 1 | UniProt (Q15843) Pfam (PF00240) | Homo sapiens (Human) | Neddylin,Neural precursor cell expressed developmentally down-regulated protein 8,NEDD-8,Ubiquitin-like protein Nedd8 |
| 5 | E (R) | E3 ubiquitin-protein ligase RBX1 | polymer | 108 | 12290.0 | 1 | UniProt (P62877) Pfam (PF12678) | Homo sapiens (Human) | E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1 |
| 6 | F (D) | Ubiquitin-conjugating enzyme E2 L3 | polymer | 154 | 17825.5 | 1 | UniProt (P68036) Pfam (PF00179) | Homo sapiens (Human) | E2 ubiquitin-conjugating enzyme L3,L-UBC,UbcH7,Ubiquitin carrier protein L3,Ubiquitin-conjugating enzyme E2-F1,Ubiquitin-protein ligase L3 |
| 7 | G, H, I, J, K... (H, R) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 8 | M (U) | 5-azanylpentan-2-one | non-polymer | 101.1 | 1 | Chemie (SY8) |
Sequence modifications
D: 1 - 154 (UniProt: P68036)
| PDB | External Database | Details |
|---|---|---|
| Ala 17 | Cys 17 | engineered mutation |
| Ala 137 | Cys 137 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 201719.9 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 689.8 | |
| All* | Total formula weight | 202409.7 |
