7B5M
Ubiquitin ligation to F-box protein substrates by SCF-RBR E3-E3 super-assembly: CUL1-RBX1-SKP1-SKP2-CKSHS1-p27~Ub~ARIH1. Transition State 2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | Cullin-1 | polymer | 776 | 89800.4 | 1 | UniProt (Q13616) Pfam (PF00888) Pfam (PF10557) | Homo sapiens (Human) | CUL-1 |
| 2 | B (H) | E3 ubiquitin-protein ligase ARIH1 | polymer | 557 | 64005.6 | 1 | UniProt (Q9Y4X5) Pfam (PF21235) Pfam (PF00097) Pfam (PF01485) Pfam (PF22191) Pfam (PF19422) | Homo sapiens (Human) | H7-AP2,HHARI,Monocyte protein 6,MOP-6,Protein ariadne-1 homolog,ARI-1,UbcH7-binding protein,UbcM4-interacting protein,Ubiquitin-conjugating enzyme E2-binding protein 1 |
| 3 | C (T) | S-phase kinase-associated protein 2 | polymer | 424 | 47817.8 | 1 | UniProt (Q13309) Pfam (PF12937) | Homo sapiens (Human) | Cyclin-A/CDK2-associated protein p45,F-box protein Skp2,F-box/LRR-repeat protein 1,p45skp2 |
| 4 | D (K) | Cyclin-dependent kinases regulatory subunit 1 | polymer | 79 | 9679.2 | 1 | UniProt (P61024) Pfam (PF01111) | Homo sapiens (Human) | CKS-1 |
| 5 | E (S) | S-phase kinase-associated protein 1 | polymer | 163 | 18680.0 | 1 | UniProt (P63208) Pfam (PF03931) Pfam (PF01466) | Homo sapiens (Human) | Cyclin-A/CDK2-associated protein p19,p19A,Organ of Corti protein 2,OCP-2,Organ of Corti protein II,OCP-II,RNA polymerase II elongation factor-like protein,SIII,Transcription elongation factor B polypeptide 1-like,p19skp1 |
| 6 | F (U) | Polyubiquitin-C | polymer | 75 | 8519.8 | 1 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (Human) | |
| 7 | G (R) | E3 ubiquitin-protein ligase RBX1 | polymer | 108 | 12290.0 | 1 | UniProt (P62877) Pfam (PF12678) | Homo sapiens (Human) | E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1 |
| 8 | H (P) | Cyclin-dependent kinase inhibitor 1B | polymer | 198 | 22185.3 | 1 | UniProt (P46527) Pfam (PF02234) | Homo sapiens (Human) | Cyclin-dependent kinase inhibitor p27,p27Kip1 |
| 9 | I, J, K, L, M (H, R) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) |
Sequence modifications
H: 1 - 557 (UniProt: Q9Y4X5)
P: 1 - 198 (UniProt: P46527)
| PDB | External Database | Details |
|---|---|---|
| Ala 430 | Phe 430 | engineered mutation |
| Ala 431 | Glu 431 | engineered mutation |
| Ala 503 | Glu 503 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Dha 163 | Gln 163 | engineered mutation |
| Arg 189 | Lys 189 | engineered mutation |
| Arg 190 | Lys 190 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 272978.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 327.0 | |
| All* | Total formula weight | 273305.0 |
