7B1A

Myosin-II-AA mutant motor domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Myosin-2 heavy chain	polymer	788	89954.5	1	UniProt (P08799) Pfam (PF02736) Pfam (PF00063)	Dictyostelium discoideum (Slime mold)	Myosin II heavy chain
2	B (D)	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900003)
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D, E, F (A)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5	G (A)	MALONATE ION	non-polymer		102.0	1	Chemie (MLI)
6	H (A)	ADP METAVANADATE	non-polymer		527.1	1	Chemie (AD9)
7	I (A)	water	water		18.0	96	Chemie (HOH)

Sequence modifications

A: 2 - 761 (UniProt: P08799)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Asp -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag
Glu 1	-	expression tag
Ala 647	Thr 647	engineered mutation
Ala 648	Thr 648	engineered mutation
Leu 762	-	expression tag
Glu 763	-	expression tag
Ser 764	-	expression tag
Asn 765	-	expression tag
Glu 766	-	expression tag
Pro 767	-	expression tag
Pro 768	-	expression tag
Met 769	-	expression tag
Asp 770	-	expression tag
Phe 771	-	expression tag
Asp 772	-	expression tag
Asp 773	-	expression tag
Asp 774	-	expression tag
Ile 775	-	expression tag
Pro 776	-	expression tag
Phe 777	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	89954.5
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	6
	Total formula weight	839.7
All*	Total formula weight	91136.5

*Water molecules are not included.