7B1A

Myosin-II-AA mutant motor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	89.764, 146.470, 154.845
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.110 - 2.600
R-factor	0.2118
Rwork	0.209
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vom
RMSD bond length	0.007
RMSD bond angle	0.794
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.110	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	31661	3109
<I/σ(I)>	13.68	1.96
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.999	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.24 M sodium malonate pH6.5, 21% PEG 3350