7AZK

DNA polymerase sliding clamp from Escherichia coli with peptide 35 bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Beta sliding clamp	polymer	386	42801.9	4	UniProt (A0A073FMV0) Pfam (PF00712) Pfam (PF02767) Pfam (PF02768) UniProt (by SIFTS) (P0A988) In PDB	Escherichia coli 2-427-07_S4_C3
2	H, I, J, K	Peptide 35	polymer	6	783.7	4		synthetic construct
3	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	A, C	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
5	A, B, C, D	PENTAETHYLENE GLYCOL	non-polymer		238.3	8	Chemie (1PE)
6	A, B, D	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
7	C, D	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
8		water	water		18.0	997	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 366 (UniProt: A0A073FMV0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	174342.4
Non-Polymers*	Number of molecules	17
	Total formula weight	2487.2
All*	Total formula weight	176829.6

*Water molecules are not included.