7AZK
DNA polymerase sliding clamp from Escherichia coli with peptide 35 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-01 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.815, 135.827, 87.541 |
| Unit cell angles | 90.00, 106.15, 90.00 |
Refinement procedure
| Resolution | 84.090 - 2.050 |
| R-factor | 0.2112 |
| Rwork | 0.210 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fvl |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 135.820 | 2.080 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rpim | 0.061 | 0.572 |
| Number of reflections | 87312 | 180 |
| <I/σ(I)> | 10.2 | 1.2 |
| Completeness [%] | 94.1 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.990 | 0.430 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.2 M Potassium thiocyanate, pH 7.0, , PEG 3350 20% (w/v) |
