7AZ6

DNA polymerase sliding clamp from Escherichia coli with peptide 36 bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Beta sliding clamp	polymer	386	42801.9	1	UniProt (A0A073FMV0) Pfam (PF00712) Pfam (PF02767) Pfam (PF02768) UniProt (by SIFTS) (P0A988) In PDB	Escherichia coli 2-427-07_S4_C3
2	H	Peptide 36	polymer	7	940.9	1		synthetic construct
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
6	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7		water	water		18.0	425	Chemie (HOH)

Sequence modifications

A: 1 - 366 (UniProt: A0A073FMV0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43742.8
Non-Polymers*	Number of molecules	5
	Total formula weight	398.8
All*	Total formula weight	44141.6

*Water molecules are not included.