7AZ6

DNA polymerase sliding clamp from Escherichia coli with peptide 36 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	108.289, 66.451, 80.272
Unit cell angles	90.00, 127.84, 90.00

Refinement procedure

Resolution	52.470 - 1.930
R-factor	0.207
Rwork	0.205
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fvl
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (3.2.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.900	1.960
High resolution limit [Å]	1.930	1.930
Rpim	0.070	0.420
Number of reflections	32437	888
<I/σ(I)>	8.5
Completeness [%]	95.8
Redundancy	11
CC(1/2)	0.990	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	2% v/v Tacsimate pH 5, 0.1M Sodium citrate tribasic dihydrate pH5.6, PEG 3350 16% (w/v)