7AUC
Crystal structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Bloom syndrome protein,Bloom syndrome protein | polymer | 562 | 63884.5 | 1 | UniProt (P54132) Pfam (PF00270) Pfam (PF00271) Pfam (PF16124) Pfam (PF00570) | Homo sapiens (Human) | DNA helicase,RecQ-like type 2,RecQ2,RecQ protein-like 3,DNA helicase,RecQ-like type 2,RecQ2,RecQ protein-like 3 |
| 2 | B, C, E, K (A) | ACETATE ION | non-polymer | 59.0 | 4 | Chemie (ACT) | |||
| 3 | D, G, H, I, J... (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
| 4 | F, N (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 2 | Chemie (PG4) | |||
| 5 | L (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | O (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 7 | P, Q (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 8 | R (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 9 | S, T (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 10 | U (A) | water | water | 18.0 | 431 | Chemie (HOH) |
Sequence modifications
A: 636 - 1196 (UniProt: P54132)
A: 1202 - 1298 (UniProt: P54132)
| PDB | External Database | Details |
|---|---|---|
| Met 611 | - | initiating methionine |
| Gly 612 | - | expression tag |
| Ser 613 | - | expression tag |
| Ala 614 | - | expression tag |
| Trp 615 | - | expression tag |
| Ser 616 | - | expression tag |
| His 617 | - | expression tag |
| Pro 618 | - | expression tag |
| Gln 619 | - | expression tag |
| Phe 620 | - | expression tag |
| Glu 621 | - | expression tag |
| Lys 622 | - | expression tag |
| Ser 623 | - | expression tag |
| Ser 624 | - | expression tag |
| Gly 625 | - | expression tag |
| Leu 626 | - | expression tag |
| Glu 627 | - | expression tag |
| Val 628 | - | expression tag |
| Leu 629 | - | expression tag |
| Phe 630 | - | expression tag |
| Gln 631 | - | expression tag |
| Gly 632 | - | expression tag |
| Pro 633 | - | expression tag |
| His 634 | - | expression tag |
| Met 635 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 1197 | - | linker |
| Ser 1198 | - | linker |
| Gly 1199 | - | linker |
| Gly 1200 | - | linker |
| Ser 1201 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 63884.5 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 1710.5 | |
| All* | Total formula weight | 65595.0 |
