7AR7
Cryo-EM structure of Arabidopsis thaliana complex-I (open conformation)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | NADH-ubiquinone oxidoreductase chain 3 | polymer | 119 | 13941.4 | 1 | UniProt (P92533) Pfam (PF00507) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 3 |
| 2 | B (B) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial | polymer | 157 | 17794.7 | 1 | UniProt (Q42577) Pfam (PF01058) | Arabidopsis thaliana (thale cress) | |
| 3 | C (C) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 | polymer | 185 | 22366.4 | 1 | UniProt (Q95748) Pfam (PF00329) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 9 |
| 4 | D (D) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 | polymer | 385 | 43889.4 | 1 | UniProt (P93306) Pfam (PF00346) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 7 |
| 5 | E (E) | NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial | polymer | 192 | 21411.4 | 1 | UniProt (O22769) Pfam (PF01257) | Arabidopsis thaliana (thale cress) | |
| 6 | F (F) | NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial | polymer | 434 | 48087.2 | 1 | UniProt (Q9FNN5) Pfam (PF01512) Pfam (PF22461) Pfam (PF10531) Pfam (PF10589) | Arabidopsis thaliana (thale cress) | |
| 7 | G (G) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial | polymer | 688 | 75100.8 | 1 | UniProt (Q9FGI6) Pfam (PF13510) Pfam (PF10588) Pfam (PF22117) Pfam (PF22151) Pfam (PF00384) Pfam (PF09326) | Arabidopsis thaliana (thale cress) | Protein EMBRYO DEFECTIVE 1467 |
| 8 | H (H) | NADH-ubiquinone oxidoreductase chain 1 | polymer | 324 | 35888.9 | 1 | UniProt (P92558) Pfam (PF00146) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 1 |
| 9 | I (I) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial | polymer | 169 | 19723.3 | 1 | UniProt (Q42599) Pfam (PF12838) | Arabidopsis thaliana (thale cress) | |
| 10 | J (J) | NADH-ubiquinone oxidoreductase chain 6 | polymer | 205 | 23690.4 | 1 | UniProt (A0A2P2CLG1) Pfam (PF00499) UniProt (by SIFTS) (P60497) | Arabidopsis thaliana (thale cress) | |
| 11 | K (K) | NADH-ubiquinone oxidoreductase chain 4L | polymer | 88 | 9892.1 | 1 | UniProt (Q04614) Pfam (PF00420) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 4L |
| 12 | L (L) | NADH-ubiquinone oxidoreductase chain 5 | polymer | 615 | 68214.8 | 1 | UniProt (P29388) Pfam (PF00662) Pfam (PF00361) Pfam (PF06455) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 5 |
| 13 | M (M) | NADH-ubiquinone oxidoreductase chain 4 | polymer | 487 | 55001.5 | 1 | UniProt (P93313) Pfam (PF00361) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 4 |
| 14 | N (N) | NADH-ubiquinone oxidoreductase chain 2 | polymer | 488 | 54161.2 | 1 | UniProt (O05000) Pfam (PF00361) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 2 |
| 15 | O (P) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial | polymer | 331 | 36400.2 | 1 | UniProt (Q9SK66) Pfam (PF01370) | Arabidopsis thaliana (thale cress) | |
| 16 | P (Q) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial | polymer | 119 | 13291.0 | 1 | UniProt (Q9FJW4) Pfam (PF04800) | Arabidopsis thaliana (thale cress) | Protein FROSTBITE1 |
| 17 | Q (R) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial | polymer | 62 | 6928.0 | 1 | UniProt (Q9M9M6) Pfam (PF10276) | Arabidopsis thaliana (thale cress) | |
| 18 | R (S) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 | polymer | 93 | 10433.2 | 1 | UniProt (Q9FIJ2) Pfam (PF05047) | Arabidopsis thaliana (thale cress) | |
| 19 | S (T) | Acyl carrier protein 1, mitochondrial | polymer | 84 | 9526.7 | 1 | UniProt (P53665) Pfam (PF00550) | Arabidopsis thaliana (thale cress) | MtACP-1,ACP,NADH-ubiquinone oxidoreductase 9.6 kDa subunit |
| 20 | T (U) | Acyl carrier protein 2, mitochondrial | polymer | 83 | 9232.3 | 1 | UniProt (O80800) Pfam (PF00550) | Arabidopsis thaliana (thale cress) | MtACP-2,ACP,NADH-ubiquinone oxidoreductase 9.6 kDa subunit |
| 21 | U (V) | Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial | polymer | 140 | 16006.2 | 1 | UniProt (Q9FLX7) Pfam (PF04716) | Arabidopsis thaliana (thale cress) | |
| 22 | V (W) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 | polymer | 133 | 15102.3 | 1 | UniProt (Q9LHI0) | Arabidopsis thaliana (thale cress) | |
| 23 | W (X) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B | polymer | 96 | 11036.9 | 1 | UniProt (Q8LGE7) Pfam (PF06747) | Arabidopsis thaliana (thale cress) | |
| 24 | X (Z) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A | polymer | 125 | 14150.1 | 1 | UniProt (Q8RWA7) Pfam (PF06212) | Arabidopsis thaliana (thale cress) | Protein MATERNAL EFFECT EMBRYO ARREST 4 |
| 25 | Y (a) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 | polymer | 58 | 6717.9 | 1 | UniProt (Q9C9Z5) Pfam (PF15879) | Arabidopsis thaliana (thale cress) | |
| 26 | Z (b) | At2g46540/F11C10.23 | polymer | 40 | 4240.2 | 1 | UniProt (Q9ZPY5) | Arabidopsis thaliana (thale cress) | Expressed protein,F11C10.23/F11C10.23,Fiber |
| 27 | AA (c) | Transmembrane protein | polymer | 76 | 8811.3 | 1 | UniProt (Q8VZT9) | Arabidopsis thaliana (thale cress) | |
| 28 | BA (d) | Excitatory amino acid transporter | polymer | 75 | 8458.0 | 1 | UniProt (Q94AL6) | Arabidopsis thaliana (thale cress) | Uncharacterized protein At4g20150 |
| 29 | CA (e) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-A | polymer | 64 | 7847.9 | 1 | UniProt (O82238) Pfam (PF10200) | Arabidopsis thaliana (thale cress) | |
| 30 | DA (f) | At4g16450 | polymer | 100 | 10720.3 | 1 | UniProt (Q84W12) Pfam (PF10785) | Arabidopsis thaliana (thale cress) | NADH-ubiquinone oxidoreductase,Uncharacterized protein At4g16450 |
| 31 | EA (g) | ESSS subunit of NADH:ubiquinone oxidoreductase (Complex I) protein | polymer | 72 | 8319.5 | 1 | UniProt (Q9SLC8) | Arabidopsis thaliana (thale cress) | |
| 32 | FA (i) | At1g67350 | polymer | 85 | 10493.9 | 1 | UniProt (Q9FYF8) Pfam (PF17250) | Arabidopsis thaliana (thale cress) | F1N21.17,NADH-ubiquinone oxidoreductase |
| 33 | GA (j) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 | polymer | 51 | 5878.9 | 1 | UniProt (Q8LDK3) | Arabidopsis thaliana (thale cress) | |
| 34 | HA (k) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A | polymer | 47 | 5337.2 | 1 | UniProt (O64725) Pfam (PF08122) | Arabidopsis thaliana (thale cress) | |
| 35 | IA (l) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial | polymer | 46 | 5074.9 | 1 | UniProt (Q9FGK0) | Arabidopsis thaliana (thale cress) | |
| 36 | JA (m) | AT2G31490 protein | polymer | 67 | 8003.2 | 1 | UniProt (Q9SIQ8) | Arabidopsis thaliana (thale cress) | At2g31490,Expressed protein,Neuronal acetylcholine receptor subunit alpha-5,Uncharacterized protein At2g31490 |
| 37 | KA (n) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 | polymer | 109 | 12864.5 | 1 | UniProt (Q945M1) | Arabidopsis thaliana (thale cress) | B22 subunit of eukaryotic mitochondrial complex I,Complex I-B22,AtCIB22,CI-B22,NADH-ubiquinone oxidoreductase B22 subunit |
| 38 | LA (o) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 | polymer | 80 | 9509.3 | 1 | UniProt (Q9SKC9) Pfam (PF05676) | Arabidopsis thaliana (thale cress) | |
| 39 | MA (p) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B | polymer | 93 | 11070.7 | 1 | UniProt (Q94C12) Pfam (PF10249) | Arabidopsis thaliana (thale cress) | |
| 40 | NA (q) | Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 | polymer | 63 | 7325.0 | 1 | UniProt (Q9M9M9) Pfam (PF05071) | Arabidopsis thaliana (thale cress) | |
| 41 | OA (r) | B14.5a | polymer | 10 | 1245.5 | 1 | Arabidopsis thaliana (thale cress) | ||
| 42 | PA (u) | unknown | polymer | 30 | 2571.2 | 1 | Arabidopsis thaliana (thale cress) | ||
| 43 | QA (v) | Uncharacterized protein At2g27730, mitochondrial | polymer | 30 | 3208.7 | 1 | UniProt (Q9ZUX4) | Arabidopsis thaliana (thale cress) | |
| 44 | RA (x) | Gamma carbonic anhydrase-like 2, mitochondrial | polymer | 214 | 23542.9 | 1 | UniProt (Q9SMN1) | Arabidopsis thaliana (thale cress) | AtCAL2,GAMMA CAL2 |
| 45 | SA (y) | Gamma carbonic anhydrase 2, mitochondrial | polymer | 268 | 28947.9 | 1 | UniProt (Q9C6B3) Pfam (PF00132) | Arabidopsis thaliana (thale cress) | AtCA2,GAMMA CA2,Transcription factor APFI |
| 46 | TA (z) | Gamma carbonic anhydrase 1, mitochondrial | polymer | 233 | 25202.6 | 1 | UniProt (Q9FWR5) | Arabidopsis thaliana (thale cress) | AtCA1,GAMMA CA1 |
| 47 | AB, CB, DB, UA, XA... (G, I, B, F) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 6 | Chemie (SF4) | |||
| 48 | VA, YA (E, G) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 49 | WA (F) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) | |||
| 50 | BB (H) | Ubiquinone-9 | non-polymer | 795.2 | 1 | Chemie (UQ9) | |||
| 51 | EB, GB, IB (I, M, N) | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 3 | Chemie (PTY) | |||
| 52 | FB, MB (M, f) | (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE | non-polymer | 763.1 | 2 | Chemie (PC7) | |||
| 53 | HB (M) | Lauryl Maltose Neopentyl Glycol | non-polymer | 1005.2 | 1 | Chemie (LMN) | |||
| 54 | JB (P) | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 1 | Chemie (NDP) | |||
| 55 | KB, OB (R, y) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 56 | LB, NB (W, n) | S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate | non-polymer | 540.7 | 2 | Chemie (8Q1) | |||
| 57 | PB (y) | (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE | non-polymer | 751.0 | 1 | Chemie (PGT) | |||
| 58 | QB (z) | 1,2-DICAPROYL-SN-PHOSPHATIDYL-L-SERINE | non-polymer | 455.4 | 1 | Chemie (PSF) | |||
| 59 | RB (z) | Phosphatidylinositol | non-polymer | 887.1 | 1 | Chemie (T7X) |
Sequence modifications
D: 10 - 394 (UniProt: P93306)
H: 2 - 325 (UniProt: P92558)
K: 1 - 88 (UniProt: Q04614)
L: 1 - 615 (UniProt: P29388)
M: 9 - 495 (UniProt: P93313)
| PDB | External Database | Details |
|---|---|---|
| Leu 70 | Ser 70 | conflict |
| Ser 227 | Pro 227 | conflict |
| Leu 309 | Ser 309 | conflict |
| Ser 363 | Leu 363 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 126 | Trp 126 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Leu 44 | Ser 44 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Phe 91 | Ser 91 | conflict |
| Phe 288 | Ser 288 | conflict |
| Leu 537 | Pro 537 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Phe 146 | Pro 146 | conflict |
| Leu 326 | Pro 326 | conflict |
| Phe 383 | Ser 383 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 46 |
| Total formula weight | 866661.7 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 12497.7 | |
| All* | Total formula weight | 879159.3 |
