7APK

Structure of the human THO - UAP56 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, I, a, i	THO complex subunit 1	polymer	711	81138.4	4	UniProt (Q96FV9) Pfam (PF11957) Pfam (PF00531) In PDB	Homo sapiens (Human)	Tho1,Nuclear matrix protein p84,p84N5,hTREX84
2	B, J, b, j	THO complex subunit 2	polymer	1226	141584.6	4	UniProt (Q8NI27) Pfam (PF16134) Pfam (PF11732) Pfam (PF11262) In PDB	Homo sapiens (Human)	Tho2,hTREX120
3	C, K, c, k	THO complex subunit 3	polymer	395	43279.4	4	UniProt (Q96J01) Pfam (PF00400) Pfam (PF12894) Pfam (PF07676) In PDB	Homo sapiens (Human)	Tho3,TEX1 homolog,hTREX45
4	E, M, e, m	THO complex subunit 5 homolog	polymer	683	78652.9	4	UniProt (Q13769) Pfam (PF09766) In PDB	Homo sapiens (Human)	Functional spliceosome-associated protein 79,fSAP79,NF2/meningioma region protein pK1.3,Placental protein 39.2,PP39.2,hTREX90
5	F, N, f, n	THO complex subunit 6 homolog	polymer	341	37577.9	4	UniProt (Q86W42) Pfam (PF00400) In PDB	Homo sapiens (Human)	Functional spliceosome-associated protein 35,fSAP35,WD repeat-containing protein 58
6	G, O, g, o	THO complex subunit 7 homolog	polymer	204	23782.0	4	UniProt (Q6I9Y2) Pfam (PF05615) In PDB	Homo sapiens (Human)	Functional spliceosome-associated protein 24,fSAP24,Ngg1-interacting factor 3-like protein 1-binding protein 1,NIF3L1-binding protein 1,hTREX30
7	H, P, h, p	Spliceosome RNA helicase DDX39B	polymer	451	51612.2	4	UniProt (Q13838) Pfam (PF00270) Pfam (PF00271) In PDB	Homo sapiens (Human)	56 kDa U2AF65-associated protein,ATP-dependent RNA helicase p47,DEAD box protein UAP56,HLA-B-associated transcript 1 protein
8	X, x	THOC2 anchor (putative)	polymer	37	3166.9	2		Homo sapiens

Sequence modifications

A, I, a, i: 2 - 657 (UniProt: Q96FV9)

PDB	External Database	Details
Met -53	-	initiating methionine
Gly -52	-	expression tag
Lys -51	-	expression tag
Pro -50	-	expression tag
Ile -49	-	expression tag
Pro -48	-	expression tag
Asn -47	-	expression tag
Pro -46	-	expression tag
Leu -45	-	expression tag
Leu -44	-	expression tag
Gly -43	-	expression tag
Leu -42	-	expression tag
Asp -41	-	expression tag
Ser -40	-	expression tag
Thr -39	-	expression tag
Gly -38	-	expression tag
Ser -37	-	expression tag
Gly -36	-	expression tag
Lys -35	-	expression tag
Pro -34	-	expression tag
Ile -33	-	expression tag
Pro -32	-	expression tag
Asn -31	-	expression tag
Pro -30	-	expression tag
Leu -29	-	expression tag
Leu -28	-	expression tag
Gly -27	-	expression tag
Leu -26	-	expression tag
Asp -25	-	expression tag
Ser -24	-	expression tag
Thr -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Lys -19	-	expression tag
Pro -18	-	expression tag
Ile -17	-	expression tag
Pro -16	-	expression tag
Asn -15	-	expression tag
Pro -14	-	expression tag
Leu -13	-	expression tag
Leu -12	-	expression tag
Gly -11	-	expression tag
Leu -10	-	expression tag
Asp -9	-	expression tag
Ser -8	-	expression tag
Thr -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Pro 1	-	expression tag

B, J, b, j: 1 - 1203 (UniProt: Q8NI27)

PDB	External Database	Details
Met -22	-	initiating methionine
Lys -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

C, K, c, k: 1 - 351 (UniProt: Q96J01)

PDB	External Database	Details
Met -43	-	initiating methionine
Lys -42	-	expression tag
Gly -41	-	expression tag
Ser -40	-	expression tag
Ala -39	-	expression tag
Trp -38	-	expression tag
Ser -37	-	expression tag
His -36	-	expression tag
Pro -35	-	expression tag
Gln -34	-	expression tag
Phe -33	-	expression tag
Glu -32	-	expression tag
Lys -31	-	expression tag
Gly -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Ser -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Ala -19	-	expression tag
Trp -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Pro -15	-	expression tag
Gln -14	-	expression tag
Phe -13	-	expression tag
Glu -12	-	expression tag
Lys -11	-	expression tag
Thr -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

E, M, e, m: 1 - 683 (UniProt: Q13769)

PDB	External Database	Details
Ile 525	Val 525	conflict
Ile 579	Val 579	conflict

H, P, h, p: 1 - 428 (UniProt: Q13838)

PDB	External Database	Details
Gly -22	-	expression tag
Pro -21	-	expression tag
Met -20	-	expression tag
Lys -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	30
	Total formula weight	1836843.3
All*	Total formula weight	1836843.3

*Water molecules are not included.