7AML

RET/GDNF/GFRa1 extracellular complex Cryo-EM structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	Proto-oncogene tyrosine-protein kinase receptor Ret	polymer	615	69536.8	2	UniProt (A8E7C6) Pfam (PF17756) Pfam (PF00028) Pfam (PF17812) Pfam (PF17813) Pfam (PF22540)	Danio rerio (Zebrafish)
2	C, E (B, E)	GDNF family receptor alpha	polymer	353	39735.2	2	UniProt (Q98TT9) Pfam (PF02351)	Danio rerio (Zebrafish)
3	D, F (C, F)	Glial cell line-derived neurotrophic factor	polymer	101	11567.2	2	UniProt (Q98TU0) Pfam (PF00019)	Danio rerio (Zebrafish)	zGDNF
4	G, H, I, J, R... (A, D)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
5	AA, BA, CA, DA, K... (D, C, F, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	16	Chemie (NAG)

Sequence modifications

A, D: 22 - 626 (UniProt: A8E7C6)

PDB	External Database	Details
Arg 505	Lys 505	conflict
Gly 627	-	expression tag
Ser 628	-	expression tag
Glu 629	-	expression tag
Phe 630	-	expression tag
Glu 631	-	expression tag
Asn 632	-	expression tag
Leu 633	-	expression tag
Tyr 634	-	expression tag
Phe 635	-	expression tag
Gln 636	-	expression tag

B, E: 20 - 351 (UniProt: Q98TT9)

PDB	External Database	Details
Gly 352	-	expression tag
Ser 353	-	expression tag
Glu 354	-	expression tag
Phe 355	-	expression tag
Leu 356	-	expression tag
Glu 357	-	expression tag
Val 358	-	expression tag
Leu 359	-	expression tag
Phe 360	-	expression tag
Gln 361	-	expression tag
Gly 362	-	expression tag
Pro 363	-	expression tag
Gly 364	-	expression tag
Gly 365	-	expression tag
Gly 366	-	expression tag
Glu 367	-	expression tag
Asn 368	-	expression tag
Leu 369	-	expression tag
Tyr 370	-	expression tag
Phe 371	-	expression tag
Gln 372	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	241678.3
Non-Polymers*	Number of molecules	24
	Total formula weight	3860.0
All*	Total formula weight	245538.3

*Water molecules are not included.