7AMF

Crystal structure of rsFolder2 in its non-fluorescent off-state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Green fluorescent protein	polymer	238	26724.1	4	UniProt (P42212) Pfam (PF01353)	Aequorea victoria
2	E, F, G (B, C, D)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	H, I, J, K (A, B, C, D)	water	water		18.0	981	Chemie (HOH)

Sequence modifications

A, B, C, D: 3 - 233 (UniProt: P42212)

PDB	External Database	Details
His -4	-	expression tag
His -3	-	expression tag
Thr -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Met 1	-	expression tag
Val 2	-	expression tag
Arg 31	Ser 30	engineered mutation
Asn 40	Tyr 39	engineered mutation
Leu 65	Phe 64	engineered mutation
Pia 68	Ser 65	chromophore
Pia 68	Tyr 66	chromophore
Pia 68	Gly 67	chromophore
Leu 70	Gln 69	engineered mutation
Arg 81	Gln 80	engineered mutation
Ser 100	Phe 99	engineered mutation
Thr 106	Asn 105	engineered mutation
Thr 154	Met 153	engineered mutation
Ser 164	Val 163	engineered mutation
Val 172	Ile 171	engineered mutation
Lys 207	Ala 206	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	106896.3
Non-Polymers*	Number of molecules	3
	Total formula weight	276.3
All*	Total formula weight	107172.6

*Water molecules are not included.