7AMF
Crystal structure of rsFolder2 in its non-fluorescent off-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-01 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.300, 134.910, 51.660 |
| Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
| Resolution | 48.120 - 1.630 |
| R-factor | 0.1551 |
| Rwork | 0.154 |
| R-free | 0.19270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dtz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.010 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC (5.8.0258) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.120 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmeas | 0.072 | 0.807 |
| Number of reflections | 116053 | 5765 |
| <I/σ(I)> | 11.3 | 1.7 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.998 | 0.679 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 3350, 100 mM Tris pH 8.5, 20 mM NaCl |
