7AKZ

Deciphering the role of the channel constrictions in the opening mechanism of MexAB-OprM efflux pump from Pseudomonas aeruginosa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Outer membrane protein OprM	polymer	474	51491.3	2	UniProt (Q51487) Pfam (PF02321)	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
2	C (A)	PALMITIC ACID	non-polymer		256.4	1	Chemie (PLM)
3	D, E, F (A)	octyl beta-D-glucopyranoside	non-polymer		292.4	3	Chemie (BOG)

Sequence modifications

A, B: 1 - 468 (UniProt: Q51487)

PDB	External Database	Details
Leu 403	Arg 420	engineered mutation
Phe 404	Tyr 421	engineered mutation
Gly 412	Leu 429	engineered mutation
Ala 416	Asp 433	engineered mutation
Ala 419	Arg 436	engineered mutation
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	102982.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1133.5
All*	Total formula weight	104116.2

*Water molecules are not included.