7AI1

Crystal structure of human MDM2-G443T RING domain homodimer bound to UbcH5B-Ub (Crystal form 2)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (AAA, DDD)	E3 ubiquitin-protein ligase Mdm2	polymer	75	8329.9	2	UniProt (Q00987) Pfam (PF13920)	Homo sapiens (Human)	Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
2	B, E (BBB, EEE)	Ubiquitin-conjugating enzyme E2 D2	polymer	147	16851.4	2	UniProt (P62837) Pfam (PF00179)	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
3	C, F (CCC, FFF)	Polyubiquitin-B	polymer	81	8922.1	2	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
4	G, L (AAA, DDD)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	H, I, M, N (AAA, DDD)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
6	J, K (BBB)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7	O, P, Q, R, S... (AAA, BBB, CCC, DDD, EEE...)	water	water		18.0	278	Chemie (HOH)

Sequence modifications

AAA, DDD: 419 - 491 (UniProt: Q00987)

PDB	External Database	Details
Gly 417	-	expression tag
Ser 418	-	expression tag
Thr 443	Gly 443	engineered mutation

BBB, EEE: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

CCC, FFF: -2 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly -4	-	expression tag
Ser -3	-	expression tag
Ser 0	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	68206.9
Non-Polymers*	Number of molecules	8
	Total formula weight	456.7
All*	Total formula weight	68663.5

*Water molecules are not included.