7AI0
Crystal structure of human MDM2-G443T RING domain homodimer bound to UbcH5B-Ub (Crystal form 1)
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | AAA, DDD | E3 ubiquitin-protein ligase Mdm2 | polymer | 75 | 8329.9 | 2 | UniProt (Q00987) Pfam (PF13920) In PDB | Homo sapiens (Human) | Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2 |
2 | BBB, EEE | Ubiquitin-conjugating enzyme E2 D2 | polymer | 147 | 16851.4 | 2 | UniProt (P62837) Pfam (PF00179) In PDB | Homo sapiens (Human) | (E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1 |
3 | CCC, FFF | Polyubiquitin-B | polymer | 81 | 8922.1 | 2 | UniProt (P0CG47) Pfam (PF00240) In PDB | Homo sapiens (Human) | |
4 | AAA, BBB, DDD | CHLORIDE ION | non-polymer | 35.5 | 5 | Chemie (CL) | |||
5 | AAA, DDD | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
6 | BBB, CCC, EEE, FFF | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
7 | water | water | 18.0 | 662 | Chemie (HOH) |
Sequence modifications
AAA, DDD: 419 - 491 (UniProt: Q00987)
BBB, EEE: 1 - 147 (UniProt: P62837)
CCC, FFF: -2 - 76 (UniProt: P0CG47)
PDB | External Database | Details |
---|---|---|
Gly 417 | - | expression tag |
Ser 418 | - | expression tag |
Thr 443 | Gly 443 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Arg 22 | Ser 22 | engineered mutation |
Lys 85 | Cys 85 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly -4 | - | expression tag |
Ser -3 | - | expression tag |
Ser 0 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 68206.9 | |
Non-Polymers* | Number of molecules | 13 |
Total formula weight | 687.2 | |
All* | Total formula weight | 68894.0 |