7AE2

Crystal structure of HEPN(H107A-Y109F) toxin in complex with MNT antitoxin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	HEPN toxin	polymer	157	18257.7	1	UniProt (A0A0B0QJR1) Pfam (PF01934)	Aphanizomenon flos-aquae 2012/KM1/D3
2	B (B)	MNT ANTITOXIN	polymer	150	17380.0	1	UniProt (A0A0B0QJN8) Pfam (PF18765)	Aphanizomenon flos-aquae 2012/KM1/D3
3	C, D (A, B)	water	water		18.0	66	Chemie (HOH)

Sequence modifications

A: 5 - 147 (UniProt: A0A0B0QJR1)

PDB	External Database	Details
Met 1	-	initiating methionine
Thr 2	-	expression tag
Asn 3	-	expression tag
Ile 4	-	expression tag
Ala 107	His 104	engineered mutation
Phe 109	Tyr 106	engineered mutation
Leu 148	-	expression tag
Glu 149	-	expression tag
Ser 150	-	expression tag
Gly 151	-	expression tag
His 152	-	expression tag
His 153	-	expression tag
His 154	-	expression tag
His 155	-	expression tag
His 156	-	expression tag
His 157	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	35637.7
All*	Total formula weight	35637.7

*Water molecules are not included.