7AD3
Class D GPCR Ste2 dimer coupled to two G proteins
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | B, A | Pheromone alpha factor receptor | polymer | 431 | 47885.4 | 2 | UniProt (P0CI39) Pfam (PF02116) UniProt (by SIFTS) (D6VTK4) In PDB | Saccharomyces cerevisiae (Baker's yeast) | |
| 2 | K, I | Alpha-factor mating pheromone | polymer | 13 | 1686.0 | 2 | Pfam (PF04648) UniProt (by SIFTS) (P01149) In PDB | Saccharomyces cerevisiae | |
| 3 | F | STE4 isoform 1 | polymer | 423 | 46627.0 | 1 | UniProt (A0A6A5Q727) Pfam (PF00400) UniProt (by SIFTS) (P18851) In PDB | Saccharomyces cerevisiae (Baker's yeast) | |
| 4 | E, H | Guanine nucleotide-binding protein alpha-1 subunit,Guanine nucleotide-binding protein alpha-1 subunit | polymer | 233 | 26316.3 | 2 | UniProt (P08539) Pfam (PF00503) In PDB | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | GP1-alpha,GP1-alpha |
| 5 | G | Guanine nucleotide-binding protein subunit gamma | polymer | 109 | 12477.1 | 1 | UniProt (A0A6A5PT44) Pfam (PF00631) UniProt (by SIFTS) (P18852) In PDB | Saccharomyces cerevisiae (Baker's yeast) | |
| 6 | B, A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 7 | B, A | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 6 | Chemie (Y01) |
Sequence modifications
E, H: 4 - 291 (UniProt: P08539)
E, H: 300 - 472 (UniProt: P08539)
G: 2 - 110 (UniProt: A0A6A5PT44)
| PDB | External Database | Details |
|---|---|---|
| Asp 50 | Gly 50 | engineered mutation |
| Asn 51 | Glu 51 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 292 | - | linker |
| Gly 293 | - | linker |
| Ser 294 | - | linker |
| Gly 295 | - | linker |
| Gly 296 | - | linker |
| Ser 297 | - | linker |
| Gly 298 | - | linker |
| Gly 299 | - | linker |
| Asp 345 | Ala 345 | engineered mutation |
| Asp 348 | Glu 348 | engineered mutation |
| - | Asp 350 | deletion |
| - | Gln 351 | deletion |
| - | Met 352 | deletion |
| - | Leu 353 | deletion |
| - | Phe 354 | deletion |
| - | Glu 355 | deletion |
| - | Asp 356 | deletion |
| - | Glu 357 | deletion |
| - | Arg 358 | deletion |
| - | Val 359 | deletion |
| Ala 450 | Met 450 | engineered mutation |
| Ile 453 | Val 453 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 106 | Cys 106 | engineered mutation |
| Ser 107 | Cys 107 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 210879.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 3362.8 | |
| All* | Total formula weight | 214242.2 |
