7AD3
Class D GPCR Ste2 dimer coupled to two G proteins
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000750 | biological_process | pheromone-dependent signal transduction involved in conjugation with cellular fusion |
| A | 0000755 | biological_process | cytogamy |
| A | 0004930 | molecular_function | G protein-coupled receptor activity |
| A | 0004932 | molecular_function | mating-type factor pheromone receptor activity |
| A | 0004934 | molecular_function | mating-type alpha-factor pheromone receptor activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0016020 | cellular_component | membrane |
| A | 0019236 | biological_process | response to pheromone |
| A | 0038038 | cellular_component | G protein-coupled receptor homodimeric complex |
| B | 0000750 | biological_process | pheromone-dependent signal transduction involved in conjugation with cellular fusion |
| B | 0000755 | biological_process | cytogamy |
| B | 0004930 | molecular_function | G protein-coupled receptor activity |
| B | 0004932 | molecular_function | mating-type factor pheromone receptor activity |
| B | 0004934 | molecular_function | mating-type alpha-factor pheromone receptor activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| B | 0016020 | cellular_component | membrane |
| B | 0019236 | biological_process | response to pheromone |
| B | 0038038 | cellular_component | G protein-coupled receptor homodimeric complex |
| E | 0003924 | molecular_function | GTPase activity |
| E | 0005525 | molecular_function | GTP binding |
| E | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| E | 0019001 | molecular_function | guanyl nucleotide binding |
| E | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| F | 0000749 | biological_process | response to pheromone triggering conjugation with cellular fusion |
| F | 0000750 | biological_process | pheromone-dependent signal transduction involved in conjugation with cellular fusion |
| F | 0001403 | biological_process | invasive growth in response to glucose limitation |
| F | 0001965 | molecular_function | G-protein alpha-subunit binding |
| F | 0005515 | molecular_function | protein binding |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005834 | cellular_component | heterotrimeric G-protein complex |
| F | 0005886 | cellular_component | plasma membrane |
| F | 0005937 | cellular_component | mating projection |
| F | 0005938 | cellular_component | cell cortex |
| F | 0007165 | biological_process | signal transduction |
| F | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| F | 0010969 | biological_process | regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion |
| F | 0019236 | biological_process | response to pheromone |
| F | 0019901 | molecular_function | protein kinase binding |
| F | 0030159 | molecular_function | signaling receptor complex adaptor activity |
| F | 0031267 | molecular_function | small GTPase binding |
| F | 0031680 | cellular_component | G-protein beta/gamma-subunit complex |
| F | 0031682 | molecular_function | G-protein gamma-subunit binding |
| F | 0043577 | biological_process | chemotropism |
| F | 0061951 | biological_process | establishment of protein localization to plasma membrane |
| F | 0097110 | molecular_function | scaffold protein binding |
| F | 0120171 | cellular_component | Cdc24p-Far1p-Gbetagamma complex |
| F | 1903260 | biological_process | protein localization to mating projection tip |
| G | 0000750 | biological_process | pheromone-dependent signal transduction involved in conjugation with cellular fusion |
| G | 0005515 | molecular_function | protein binding |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005834 | cellular_component | heterotrimeric G-protein complex |
| G | 0005886 | cellular_component | plasma membrane |
| G | 0005938 | cellular_component | cell cortex |
| G | 0007165 | biological_process | signal transduction |
| G | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| G | 0010969 | biological_process | regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion |
| G | 0016020 | cellular_component | membrane |
| G | 0019236 | biological_process | response to pheromone |
| G | 0031680 | cellular_component | G-protein beta/gamma-subunit complex |
| G | 0031681 | molecular_function | G-protein beta-subunit binding |
| G | 0071944 | cellular_component | cell periphery |
| G | 0120171 | cellular_component | Cdc24p-Far1p-Gbetagamma complex |
| H | 0003924 | molecular_function | GTPase activity |
| H | 0005525 | molecular_function | GTP binding |
| H | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| H | 0019001 | molecular_function | guanyl nucleotide binding |
| H | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| I | 0000772 | molecular_function | mating pheromone activity |
| I | 0005576 | cellular_component | extracellular region |
| I | 0019953 | biological_process | sexual reproduction |
| K | 0000772 | molecular_function | mating pheromone activity |
| K | 0005576 | cellular_component | extracellular region |
| K | 0019953 | biological_process | sexual reproduction |
Functional Information from PROSITE/UniProt
| site_id | PS00678 |
| Number of Residues | 15 |
| Details | WD_REPEATS_1 Trp-Asp (WD) repeats signature. ILSAsqDgFMLIWDS |
| Chain | Residue | Details |
| F | ILE107-SER121 | |
| F | ILE195-ILE209 | |
| F | MET364-VAL378 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | MOD_RES: Cysteine methyl ester => ECO:0000269|PubMed:10523659 |
| Chain | Residue | Details |
| A | PHE244-TYR266 | |
| A | VAL276-ALA298 | |
| E | SER55 | |
| E | THR300 | |
| E | ASP319 | |
| E | LYS398 | |
| E | LEU454 | |
| H | GLY48 | |
| H | SER55 | |
| H | THR300 | |
| H | ASP319 | |
| H | LYS398 | |
| H | LEU454 | |
| G | SER107 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:10523659, ECO:0000269|PubMed:10712512 |
| Chain | Residue | Details |
| G | SER106 | |
| B | SER360 | |
| B | SER385 | |
| A | SER310 | |
| A | SER360 | |
| A | SER385 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | LIPID: S-farnesyl cysteine => ECO:0000269|PubMed:10523659, ECO:0000269|PubMed:10712512 |
| Chain | Residue | Details |
| G | SER107 | |
| A | SER315 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| A | THR329 | |
| A | THR382 | |
| B | THR329 | |
| B | THR382 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| B | SER386 | |
| A | SER386 | |
| B | SER331 | |
| A | SER331 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:17330950 |
| Chain | Residue | Details |
| B | THR363 | |
| B | THR411 | |
| B | THR414 | |
| A | THR363 | |
| A | THR411 | |
| A | THR414 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:15665377, ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198 |
| Chain | Residue | Details |
| B | SER366 | |
| A | SER366 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:11583169 |
| Chain | Residue | Details |
| B | ASN25 | |
| B | ASN32 | |
| A | ASN25 | |
| A | ASN32 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
| Chain | Residue | Details |
| B | LYS374 | |
| B | LYS400 | |
| B | LYS422 | |
| A | LYS374 | |
| A | LYS400 | |
| A | LYS422 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 202 |
| Details | TOPO_DOM: Extracellular => ECO:0000305|PubMed:1653030, ECO:0000305|PubMed:2017168 |
| Chain | Residue | Details |
| B | MET1-VAL49 | |
| B | LEU103-ASN132 | |
| B | MET189-ASN205 | |
| B | SER267-ASP275 | |
| A | MET1-VAL49 | |
| A | LEU103-ASN132 | |
| A | MET189-ASN205 | |
| A | SER267-ASP275 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 44 |
| Details | TRANSMEM: Helical; Name=1 => ECO:0000269|PubMed:11112532, ECO:0000269|PubMed:11467956, ECO:0000269|PubMed:19383463, ECO:0007744|PDB:2K9P |
| Chain | Residue | Details |
| B | THR50-THR72 | |
| A | THR50-THR72 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 304 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000305|PubMed:1653030, ECO:0000305|PubMed:2017168 |
| Chain | Residue | Details |
| B | SER73-THR78 | |
| B | ASP157-GLY163 | |
| B | ARG231-PHE241 | |
| B | ASN300-LEU431 | |
| A | SER73-THR78 | |
| A | ASP157-GLY163 | |
| A | ARG231-PHE241 | |
| A | ASN300-LEU431 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 46 |
| Details | TRANSMEM: Helical; Name=2 => ECO:0000269|PubMed:11112532, ECO:0000269|PubMed:11467956, ECO:0000269|PubMed:19383463, ECO:0007744|PDB:2K9P |
| Chain | Residue | Details |
| B | PRO79-LEU102 | |
| A | PRO79-LEU102 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 46 |
| Details | TRANSMEM: Helical; Name=3 => ECO:0000269|PubMed:11112532, ECO:0000269|PubMed:11467956 |
| Chain | Residue | Details |
| B | ILE133-GLY156 | |
| A | ILE133-GLY156 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 48 |
| Details | TRANSMEM: Helical; Name=4 => ECO:0000269|PubMed:11112532, ECO:0000269|PubMed:11467956 |
| Chain | Residue | Details |
| B | LEU164-GLY188 | |
| A | LEU164-GLY188 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 48 |
| Details | TRANSMEM: Helical; Name=5 => ECO:0000269|PubMed:11112532, ECO:0000269|PubMed:11467956 |
| Chain | Residue | Details |
| B | ALA206-ILE230 | |
| A | ALA206-ILE230 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 48 |
| Details | TRANSMEM: Helical; Name=6 => ECO:0000269|PubMed:11112532, ECO:0000269|PubMed:11467956, ECO:0000269|PubMed:11473349, ECO:0007744|PDB:1PJD |
| Chain | Residue | Details |
| B | ASP242-TYR266 | |
| A | ASP242-TYR266 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 46 |
| Details | TRANSMEM: Helical; Name=7 => ECO:0000269|PubMed:11112532, ECO:0000269|PubMed:11467956 |
| Chain | Residue | Details |
| B | VAL276-ALA299 | |
| A | VAL276-ALA299 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:9659916 |
| Chain | Residue | Details |
| B | LYS337 | |
| A | LYS337 |
