7ABR

Cryo-EM structure of B. subtilis ClpC (DWB mutant) hexamer bound to a substrate polypeptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Negative regulator of genetic competence ClpC/MecB	polymer	818	91206.8	6	UniProt (P37571) Pfam (PF02861) Pfam (PF00004) Pfam (PF17871) Pfam (PF07724) Pfam (PF10431) In PDB	Bacillus subtilis (strain 168)
2	S	substrate polypeptide	polymer	26	2230.7	1		unidentified
3	A, B, F	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
4	A, C, D, E	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	7	Chemie (ATP)

Sequence modifications

A, B, C, D, E, F: 1 - 810 (UniProt: P37571)

PDB	External Database	Details
Ala 280	Glu 280	engineered mutation
Ala 618	Glu 618	engineered mutation
Leu 811	-	expression tag
Glu 812	-	expression tag
His 813	-	expression tag
His 814	-	expression tag
His 815	-	expression tag
His 816	-	expression tag
His 817	-	expression tag
His 818	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	549471.6
Non-Polymers*	Number of molecules	11
	Total formula weight	5259.1
All*	Total formula weight	554730.7

*Water molecules are not included.