7A5V

CryoEM structure of a human gamma-aminobutyric acid receptor, the GABA(A)R-beta3 homopentamer, in complex with histamine and megabody Mb25 in lipid nanodisc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3	polymer	396	45744.3	1	UniProt (P28472) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (Human)	GABA(A) receptor subunit beta-3,GABA(A) receptor subunit beta-3
2	B (O)	Megabody Mb25	polymer	522	56300.6	1		Lama glama (Llama)
3	C (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	D (C)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
5	E (A)	HEXADECANE	non-polymer		226.4	1	Chemie (R16)
6	F, G, H, J (A)	DECANE	non-polymer		142.3	4	Chemie (D10)
7	I (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
8	K (A)	N-OCTANE	non-polymer		114.2	1	Chemie (OCT)
9	L (A)	HISTAMINE	non-polymer		111.1	1	Chemie (HSM)
10	M, N (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
11	O, P (A, O)	water	water		18.0	203	Chemie (HOH)

Sequence modifications

A: 1 - 307 (UniProt: P28472)

PDB	External Database	Details
Glu -54	-	expression tag
Met -53	-	expression tag
Asp -52	-	expression tag
Glu -51	-	expression tag
Lys -50	-	expression tag
Thr -49	-	expression tag
Thr -48	-	expression tag
Gly -47	-	expression tag
Trp -46	-	expression tag
Arg -45	-	expression tag
Gly -44	-	expression tag
Gly -43	-	expression tag
His -42	-	expression tag
Val -41	-	expression tag
Val -40	-	expression tag
Glu -39	-	expression tag
Gly -38	-	expression tag
Leu -37	-	expression tag
Ala -36	-	expression tag
Gly -35	-	expression tag
Glu -34	-	expression tag
Leu -33	-	expression tag
Glu -32	-	expression tag
Gln -31	-	expression tag
Leu -30	-	expression tag
Arg -29	-	expression tag
Ala -28	-	expression tag
Arg -27	-	expression tag
Leu -26	-	expression tag
Glu -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Pro -22	-	expression tag
Gln -21	-	expression tag
Gly -20	-	expression tag
Gln -19	-	expression tag
Arg -18	-	expression tag
Glu -17	-	expression tag
Pro -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Thr -1	-	expression tag
Gly 0	-	expression tag
Thr 279	-	insertion

A: 422 - 448 (UniProt: P28472)

PDB	External Database	Details
Ser 308	-	linker
Gln 309	-	linker
Pro 310	-	linker
Ala 311	-	linker
Arg 312	-	linker
Ala 313	-	linker
Ala 314	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	102044.9
Branched	Number of molecules	2
	Total formula weight	1335.2
Non-Polymers*	Number of molecules	10
	Total formula weight	1313.1
All*	Total formula weight	104693.2

*Water molecules are not included.