7A0Y

Structure of dimeric sodium proton antiporter NhaA K300R variant, at pH 8.2, crystallized with chimeric Fab antibodies

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Na(+)/H(+) antiporter NhaA	polymer	411	44047.3	2	UniProt (Q8ZRZ3) Pfam (PF06965) In PDB	Salmonella enterica subsp. enterica serovar Typhimurium str. LT2	Sodium/proton antiporter NhaA
2	C, E	chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain	polymer	252	26305.0	2		Gallus gallus (Chicken)
3	D, F	chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain	polymer	210	22393.8	2		Gallus gallus (Chicken)
4	A	CARDIOLIPIN	non-polymer		1464.0	1	Chemie (CDL)
5	A, B	1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE	non-polymer		705.0	3	Chemie (3PH)
6	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
7	A, B	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
8	B	1,2-Distearoyl-sn-glycerophosphoethanolamine	non-polymer		748.1	1	Chemie (3PE)
9	C, E	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
10		water	water		18.0	147	Chemie (HOH)

Sequence modifications

A, B: 2 - 388 (UniProt: Q8ZRZ3)

PDB	External Database	Details
Met -4	-	initiating methionine
Asn -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ile 0	-	expression tag
Leu 1	-	expression tag
Arg 300	Lys 300	engineered mutation
Ser 389	-	expression tag
Glu 390	-	expression tag
Asn 391	-	expression tag
Leu 392	-	expression tag
Tyr 393	-	expression tag
Phe 394	-	expression tag
Gln 395	-	expression tag
Gly 396	-	expression tag
Gly 397	-	expression tag
Arg 398	-	expression tag
Gly 399	-	expression tag
Ser 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag

C, E: 1 - 144 (PDB: 7A0Y)
C, E: 145 - 252 (PDB: 7A0Y)
D, F: 1 - 124 (PDB: 7A0Y)
D, F: 125 - 210 (PDB: 7A0Y)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	185492.2
Non-Polymers*	Number of molecules	13
	Total formula weight	5047.3
All*	Total formula weight	190539.5

*Water molecules are not included.