6ZZ6
Cryo-EM structure of S.cerevisiae cohesin-Scc2-DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Structural maintenance of chromosomes protein 1,Structural maintenance of chromosomes protein 1,Structural maintenance of chromosomes protein 1 | polymer | 360 | 40854.5 | 1 | UniProt (P32908) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | DA-box protein SMC1,DA-box protein SMC1,DA-box protein SMC1 |
| 2 | B (B) | Structural maintenance of chromosomes protein 3,Structural maintenance of chromosomes protein 3,Structural maintenance of chromosomes protein 3 | polymer | 423 | 48295.0 | 1 | UniProt (P47037) Pfam (PF02463) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | DA-box protein SMC3,DA-box protein SMC3,DA-box protein SMC3 |
| 3 | C (C) | Sister chromatid cohesion protein 1,Sister chromatid cohesion protein 1,Sister chromatid cohesion protein 1 | polymer | 83 | 9123.6 | 1 | UniProt (Q12158) Pfam (PF04825) Pfam (PF04824) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 4 | D (D) | Sister chromatid cohesion protein 2 | polymer | 1493 | 171310.6 | 1 | UniProt (Q04002) Pfam (PF12765) Pfam (PF12830) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 5 | E (F) | DNA (34-MER) | polymer | 34 | 10604.1 | 1 | synthetic construct | ||
| 6 | F (G) | DNA (34-MER) | polymer | 34 | 10297.6 | 1 | synthetic construct | ||
| 7 | G, I (A, B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 8 | H, J (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
A: 2 - 71 (UniProt: P32908)
A: 87 - 195 (UniProt: P32908)
A: 1044 - 1224 (UniProt: P32908)
B: 2 - 228 (UniProt: P47037)
B: 997 - 1071 (UniProt: P47037)
B: 1104 - 1222 (UniProt: P47037)
C: 67 - 103 (UniProt: Q12158)
C: 502 - 510 (UniProt: Q12158)
C: 519 - 555 (UniProt: Q12158)
A: 87 - 195 (UniProt: P32908)
A: 1044 - 1224 (UniProt: P32908)
| PDB | External Database | Details |
|---|---|---|
| Gln 1158 | Glu 1158 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
B: 1104 - 1222 (UniProt: P47037)
| PDB | External Database | Details |
|---|---|---|
| Gln 1155 | Glu 1155 | conflict |
C: 502 - 510 (UniProt: Q12158)
C: 519 - 555 (UniProt: Q12158)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 290485.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1063.0 | |
| All* | Total formula weight | 291548.4 |
