6ZXC

Diguanylate cyclase DgcR (I-site mutant) in activated state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Putative GGDEF/response regulator receiver domain protein	polymer	318	36026.0	4	UniProt (B0SUI1) Pfam (PF00072) Pfam (PF00990)	Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris)
2	E, G, H, I, K... (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	12	Chemie (MG)
3	F, J, N, R (A, B, C, D)	3'-DEOXY-GUANOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	4	Chemie (GH3)
4	U, V, W, X (A, B, C, D)	water	water		18.0	33	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 298 (UniProt: B0SUI1)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 206	Arg 206	engineered mutation
Ala 209	Asp 209	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	144103.9
Non-Polymers*	Number of molecules	16
	Total formula weight	2320.4
All*	Total formula weight	146424.3

*Water molecules are not included.