6ZQT
Crystal structure of the RLIP76 Ral binding domain mutant (E427H/Q433L/K440R) in complex with RalB-GMPPNP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Ras-related protein Ral-B | polymer | 185 | 20998.7 | 2 | UniProt (P11234) Pfam (PF00071) In PDB | Homo sapiens (Human) | |
2 | C, D | RalA-binding protein 1 | polymer | 59 | 6890.0 | 2 | UniProt (Q15311) Pfam (PF20924) In PDB | Homo sapiens (Human) | RalBP1,76 kDa Ral-interacting protein,Dinitrophenyl S-glutathione ATPase,DNP-SG ATPase,Ral-interacting protein 1 |
3 | A, B | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | non-polymer | 522.2 | 2 | Chemie (GNP) | |||
4 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
5 | C | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
6 | water | water | 18.0 | 361 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 185 (UniProt: P11234)
C, D: 393 - 446 (UniProt: Q15311)
PDB | External Database | Details |
---|---|---|
Leu 72 | Gln 72 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 388 | - | expression tag |
Pro 389 | - | expression tag |
Leu 390 | - | expression tag |
Gly 391 | - | expression tag |
Ser 392 | - | expression tag |
Ser 411 | Cys 411 | engineered mutation |
His 427 | Glu 427 | engineered mutation |
Leu 433 | Gln 433 | engineered mutation |
Arg 440 | Lys 440 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 55777.3 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 1185.1 | |
All* | Total formula weight | 56962.4 |