6ZQT
Crystal structure of the RLIP76 Ral binding domain mutant (E427H/Q433L/K440R) in complex with RalB-GMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-08-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.450, 77.430, 66.400 |
Unit cell angles | 90.00, 90.31, 90.00 |
Refinement procedure
Resolution | 50.404 - 1.510 |
R-factor | 0.1901 |
Rwork | 0.189 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2kwi |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.404 | 50.400 | 1.550 |
High resolution limit [Å] | 1.510 | 6.750 | 1.510 |
Rmerge | 0.061 | 0.055 | 1.056 |
Rmeas | 0.065 | 0.059 | 1.195 |
Rpim | 0.022 | 0.020 | 0.552 |
Total number of observations | 7159 | 24662 | |
Number of reflections | 75045 | 871 | 5476 |
<I/σ(I)> | 18.3 | 68.2 | 1.2 |
Completeness [%] | 99.7 | 99.3 | 98.7 |
Redundancy | 7.6 | 8.2 | 4.5 |
CC(1/2) | 0.999 | 0.993 | 0.624 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 0.1 M Bicine 9.0 pH, 30% w/v PEG 6000 |