6ZOZ

Structure of Disulphide-stabilized SARS-CoV-2 Spike Protein Trimer (x1 disulphide-bond mutant, S383C, D985C, K986P, V987P, single Arg S1/S2 cleavage site) in Locked State

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Spike glycoprotein	polymer	1247	138282.9	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB	Severe acute respiratory syndrome coronavirus 2	S glycoprotein,E2,Peplomer protein
2	D, E, F, G, H...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	12	In PDB GlyTouCan (G42666HT)
3	A, C, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	39	Chemie (NAG)
4	A, C, B	BILIVERDINE IX ALPHA	non-polymer		582.6	3	Chemie (BLA)

Sequence modifications

A, B, C: 14 - 1207 (UniProt: P0DTC2)

PDB	External Database	Details
Glu 10	-	expression tag
Thr 11	-	expression tag
Gly 12	-	expression tag
Thr 13	-	expression tag
Cys 383	Ser 383	engineered mutation
-	Pro 681	deletion
-	Arg 682	deletion
-	Arg 683	deletion
-	Ala 684	deletion
Cys 981	Asp 985	engineered mutation
Pro 982	Lys 986	engineered mutation
Pro 983	Val 987	engineered mutation
Gly 1208	-	expression tag
Ser 1209	-	expression tag
Gly 1210	-	expression tag
Arg 1211	-	expression tag
Glu 1212	-	expression tag
Asn 1213	-	expression tag
Leu 1214	-	expression tag
Tyr 1215	-	expression tag
Phe 1216	-	expression tag
Gln 1217	-	expression tag
Gly 1218	-	expression tag
Gly 1219	-	expression tag
Gly 1220	-	expression tag
Gly 1221	-	expression tag
Ser 1222	-	expression tag
Gly 1223	-	expression tag
Tyr 1224	-	expression tag
Ile 1225	-	expression tag
Pro 1226	-	expression tag
Glu 1227	-	expression tag
Ala 1228	-	expression tag
Pro 1229	-	expression tag
Arg 1230	-	expression tag
Asp 1231	-	expression tag
Gly 1232	-	expression tag
Gln 1233	-	expression tag
Ala 1234	-	expression tag
Tyr 1235	-	expression tag
Val 1236	-	expression tag
Arg 1237	-	expression tag
Lys 1238	-	expression tag
Asp 1239	-	expression tag
Gly 1240	-	expression tag
Glu 1241	-	expression tag
Trp 1242	-	expression tag
Val 1243	-	expression tag
Leu 1244	-	expression tag
Leu 1245	-	expression tag
Ser 1246	-	expression tag
Thr 1247	-	expression tag
Phe 1248	-	expression tag
Leu 1249	-	expression tag
Gly 1250	-	expression tag
His 1251	-	expression tag
His 1252	-	expression tag
His 1253	-	expression tag
His 1254	-	expression tag
His 1255	-	expression tag
His 1256	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	414848.8
Branched	Number of molecules	12
	Total formula weight	5092.8
Non-Polymers*	Number of molecules	42
	Total formula weight	10375.0
All*	Total formula weight	430316.7

*Water molecules are not included.