6ZOZ
Structure of Disulphide-stabilized SARS-CoV-2 Spike Protein Trimer (x1 disulphide-bond mutant, S383C, D985C, K986P, V987P, single Arg S1/S2 cleavage site) in Locked State
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Quantum LS |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.5 |
Number of Particle | 587351 |
Software | RELION,SerialEM,CTFFIND,Coot,PHENIX |
Symmetry | C3 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |