6ZMD
Crystal structure of HYPE covalently tethered to BiP bound to AMP-PNP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Endoplasmic reticulum chaperone BiP | polymer | 527 | 57827.4 | 1 | UniProt (P11021) Pfam (PF00012) In PDB | Homo sapiens (Human) | 78 kDa glucose-regulated protein,GRP-78,Binding-immunoglobulin protein,BiP,Heat shock protein 70 family protein 5,HSP70 family protein 5,Heat shock protein family A member 5,Immunoglobulin heavy chain-binding protein |
2 | B | Protein adenylyltransferase FICD | polymer | 344 | 39392.1 | 1 | UniProt (Q9BVA6) Pfam (PF02661) In PDB | Homo sapiens (Human) | AMPylator FICD,De-AMPylase FICD,FIC domain-containing protein,Huntingtin yeast partner E,Huntingtin-interacting protein 13,HIP-13,Huntingtin-interacting protein E |
3 | A | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
4 | A | ~{N}-[2-[1-[(2~{R},3~{R},4~{S},5~{R})-3,4-bis(oxidanyl)-5-[[tris(oxidanyl)-$l^{5}-phosphanyl]oxymethyl]oxolan-2-yl]-1,2,3-triazol-4-yl]ethyl]ethanamide | non-polymer | 366.3 | 1 | Chemie (QMK) | |||
5 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
6 | A, B | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
7 | water | water | 18.0 | 74 | Chemie (HOH) |
Sequence modifications
A: 28 - 549 (UniProt: P11021)
B: 102 - 445 (UniProt: Q9BVA6)
PDB | External Database | Details |
---|---|---|
Gly 23 | - | expression tag |
Ala 24 | - | expression tag |
Met 25 | - | expression tag |
Gly 26 | - | expression tag |
Ser 27 | - | expression tag |
Ala 229 | Thr 229 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ala 168 | Thr 168 | engineered mutation |
Ala 183 | Thr 183 | engineered mutation |
Gly 234 | Glu 234 | engineered mutation |
Asp 258 | Leu 258 | engineered mutation |
Cys 404 | Glu 404 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 97219.5 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 1111.0 | |
All* | Total formula weight | 98330.5 |