6ZMD

Crystal structure of HYPE covalently tethered to BiP bound to AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976250
Spacegroup name	P 41
Unit cell lengths	83.466, 83.466, 169.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.730 - 2.640
R-factor	0.1944
Rwork	0.192
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u04 5o4p
RMSD bond length	0.002
RMSD bond angle	0.390
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MoRDa
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.470	2.770
High resolution limit [Å]	2.640	2.640
Rmerge	0.057	0.567
Number of reflections	34024	4518
<I/σ(I)>	10.1	1.9
Completeness [%]	100.0
Redundancy	3.7
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M NaCl, 0.1 M Na/K phosphate pH 6.2 and 10% (w/v) PEG 8000