6ZEI

Structure of PP1-IRSp53 S455E chimera [PP1(7-304) + linker (G/S)x9 + IRSp53(449-465)] bound to Phactr1 (516-580)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit,Brain-specific angiogenesis inhibitor 1-associated protein 2	polymer	338	37720.8	2	UniProt (P62136) UniProt (Q9UQB8) Pfam (PF16891) Pfam (PF00149) In PDB	Homo sapiens (Human)	PP-1A,Protein BAP2,Fas ligand-associated factor 3,FLAF3,Insulin receptor substrate p53/p58,IRSp53/58,Insulin receptor substrate protein of 53 kDa,Insulin receptor substrate p53
2	C, D	Phosphatase and actin regulator	polymer	70	8257.3	2	UniProt (Q4VY12) UniProt (by SIFTS) (Q9C0D0) In PDB	Homo sapiens (Human)
3	A, B	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
4	A, B	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
6		water	water		18.0	691	Chemie (HOH)

Sequence modifications

A, B: 7 - 304 (UniProt: P62136)

PDB	External Database	Details
Gly 2	-	expression tag
His 3	-	expression tag
Met 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag
Ser 305	-	linker
Gly 306	-	linker
Ser 307	-	linker
Gly 308	-	linker
Ser 309	-	linker
Gly 310	-	linker
Ser 311	-	linker
Gly 312	-	linker
Ser 313	-	linker
Gly 314	-	linker
Ser 315	-	linker
Gly 316	-	linker
Ser 317	-	linker
Gly 318	-	linker
Ser 319	-	linker
Gly 320	-	linker
Ser 321	-	linker
Gly 322	-	linker

A, B: 323 - 339 (UniProt: Q9UQB8)

PDB	External Database	Details
Glu 329	Ser 454	conflict

C, D: 516 - 580 (UniProt: Q4VY12)

PDB	External Database	Details
Gly 511	-	expression tag
Pro 512	-	expression tag
Leu 513	-	expression tag
Gly 514	-	expression tag
Ser 515	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	91956.2
Non-Polymers*	Number of molecules	9
	Total formula weight	686.0
All*	Total formula weight	92642.2

*Water molecules are not included.