6ZEI

Structure of PP1-IRSp53 S455E chimera [PP1(7-304) + linker (G/S)x9 + IRSp53(449-465)] bound to Phactr1 (516-580)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	48.795, 122.329, 69.424
Unit cell angles	90.00, 92.27, 90.00

Refinement procedure

Resolution	69.370 - 1.390
R-factor	0.1299
Rwork	0.130
R-free	0.15790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mov
RMSD bond length	0.011
RMSD bond angle	1.287
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.370	1.440
High resolution limit [Å]	1.390	1.390
Rmerge	0.065	1.050
Rmeas	0.071	1.180
Rpim	0.027	0.520
Number of reflections	162314	15982
<I/σ(I)>	12.54	1.16
Completeness [%]	99.6
Redundancy	6.5
CC(1/2)	0.999	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	293	20% (w/v) polyethylene glycol 3350 and 0.2 M NaBr