6ZDM

Crystal structure of human heparanase in complex with a N',6O'-bis-sulfated 4-methylumbelliferyl heparan sulfate disaccharide

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (AAA)	Heparanase	polymer	389	43733.3	1	UniProt (Q9Y251) Pfam (PF03662)	Homo sapiens (Human)	Endo-glucoronidase,Heparanase-1,Hpa1
2	B (BBB)	Heparanase	polymer	77	8542.8	1	UniProt (Q9Y251)	Homo sapiens (Human)	Endo-glucoronidase,Heparanase-1,Hpa1
3	C (AAA)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
4	D (AAA)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	E (AAA)	(2~{S},3~{S},4~{R},5~{R},6~{S})-3-[(2~{R},3~{R},4~{R},5~{S},6~{R})-4,5-bis(oxidanyl)-3-(sulfoamino)-6-(sulfooxymethyl)oxan-2-yl]oxy-6-(4-methyl-2-oxidanylidene-chromen-7-yl)oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid	non-polymer		673.6	1	Chemie (QG2)
6	F, G, H, I, J (AAA)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
7	K, L (AAA, BBB)	water	water		18.0	191	Chemie (HOH)

Sequence modifications

AAA: 158 - 543 (UniProt: Q9Y251)

PDB	External Database	Details
Asp 155	-	expression tag
Pro 156	-	expression tag
Gly 157	-	expression tag
Arg 307	Lys 307	conflict

BBB: 1 - 74 (UniProt: Q9Y251)

PDB	External Database	Details
Asp -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52276.1
Non-Polymers*	Number of molecules	8
	Total formula weight	1437.2
All*	Total formula weight	53713.3

*Water molecules are not included.