6ZCE
Structure of a yeast ABCE1-bound 43S pre-initiation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (l) | Eukaryotic translation initiation factor 3 subunit I | polymer | 347 | 38803.4 | 1 | UniProt (P40217) Pfam (PF00400) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | eIF3i,Eukaryotic translation initiation factor 3 39 kDa subunit,eIF3 p39 |
| 2 | B (r) | Eukaryotic translation initiation factor 3 subunit G | polymer | 274 | 30520.5 | 1 | UniProt (Q04067) Pfam (PF12353) Pfam (PF00076) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | eIF3g,Eukaryotic translation initiation factor 3 RNA-binding subunit,eIF-3 RNA-binding subunit,Translation initiation factor eIF3 p33 subunit,eIF3 p33 |
| 3 | C (A) | 18S ribosomal RNA (1719-MER) | polymer | 1800 | 579761.9 | 1 | GenBank (874346701) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | |
| 4 | D (B) | 40S ribosomal protein S0-A | polymer | 252 | 28051.3 | 1 | UniProt (P32905) Pfam (PF00318) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Nucleic acid-binding protein NAB1A,Small ribosomal subunit protein uS2-A |
| 5 | E (C) | 40S ribosomal protein S1-A | polymer | 255 | 28798.5 | 1 | UniProt (P33442) Pfam (PF01015) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP10A,Small ribosomal subunit protein eS1-A |
| 6 | F (D) | 40S ribosomal protein S2 | polymer | 254 | 27490.8 | 1 | UniProt (P25443) Pfam (PF00333) Pfam (PF03719) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Omnipotent suppressor protein SUP44,RP12,S4,Small ribosomal subunit protein uS5,YS5 |
| 7 | G (E) | 40S ribosomal protein S3 | polymer | 240 | 26542.8 | 1 | UniProt (P05750) Pfam (PF07650) Pfam (PF00189) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP13,Small ribosomal subunit protein uS3,YS3 |
| 8 | H (F) | 40S ribosomal protein S4-A | polymer | 261 | 29469.3 | 1 | UniProt (P0CX35) Pfam (PF00900) Pfam (PF00467) Pfam (PF16121) Pfam (PF08071) Pfam (PF01479) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP5,S7,Small ribosomal subunit protein eS4-A,YS6 |
| 9 | I (G) | 40S ribosomal protein S5 | polymer | 225 | 25072.6 | 1 | UniProt (P26783) Pfam (PF00177) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP14,S2,Small ribosomal subunit protein uS7,YS8 |
| 10 | J (H) | 40S ribosomal protein S6-A | polymer | 236 | 27054.5 | 1 | UniProt (P0CX37) Pfam (PF01092) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP9,S10,Small ribosomal subunit protein eS6-A,YS4 |
| 11 | K (I) | 40S ribosomal protein S7-A | polymer | 190 | 21658.2 | 1 | UniProt (P26786) Pfam (PF01251) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP30,RP40,Small ribosomal subunit protein eS7-A |
| 12 | L (J) | 40S ribosomal protein S8-A | polymer | 200 | 22537.8 | 1 | UniProt (P0CX39) Pfam (PF01201) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP19,S14,Small ribosomal subunit protein eS8-A,YS9 |
| 13 | M (K) | 40S ribosomal protein S9-A | polymer | 197 | 22487.9 | 1 | UniProt (O13516) Pfam (PF00163) Pfam (PF01479) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP21,S13,Small ribosomal subunit protein uS4-A,YP28,YS11 |
| 14 | N (L) | 40S ribosomal protein S10-A | polymer | 105 | 12757.4 | 1 | UniProt (Q08745) Pfam (PF03501) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Small ribosomal subunit protein eS10-A |
| 15 | O (M) | 40S ribosomal protein S11-A | polymer | 155 | 17668.8 | 1 | UniProt (P0CX47) Pfam (PF16205) Pfam (PF00366) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP41,S18,Small ribosomal subunit protein uS17-A,YS12 |
| 16 | P (N) | 40S ribosomal protein S12 | polymer | 143 | 15488.6 | 1 | UniProt (P48589) Pfam (PF01248) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Small ribosomal subunit protein eS12 |
| 17 | Q (O) | 40S ribosomal protein S13 | polymer | 151 | 17059.9 | 1 | UniProt (P05756) Pfam (PF08069) Pfam (PF00312) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | S27a,Small ribosomal subunit protein uS15,YS15 |
| 18 | R (P) | 40S ribosomal protein S14-A | polymer | 136 | 14431.5 | 1 | UniProt (P06367) Pfam (PF00411) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP59A,Small ribosomal subunit protein uS11-A |
| 19 | S (Q) | 40S ribosomal protein S15 | polymer | 141 | 15830.6 | 1 | UniProt (Q01855) Pfam (PF00203) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RIG protein,RP52,S21,Small ribosomal subunit protein uS19,YS21 |
| 20 | T (R) | 40S ribosomal protein S16-A | polymer | 143 | 15877.5 | 1 | UniProt (P0CX51) Pfam (PF00380) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP61R,Small ribosomal subunit protein uS9-A |
| 21 | U (S) | 40S ribosomal protein S17-A | polymer | 136 | 15820.4 | 1 | UniProt (P02407) Pfam (PF00833) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP51A,Small ribosomal subunit protein eS17-A |
| 22 | V (T) | 40S ribosomal protein S18-A | polymer | 146 | 17071.6 | 1 | UniProt (P0CX55) Pfam (PF00416) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Small ribosomal subunit protein uS13-A |
| 23 | W (U) | 40S ribosomal protein S19-A | polymer | 144 | 15942.1 | 1 | UniProt (P07280) Pfam (PF01090) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP55A,S16a,Small ribosomal subunit protein eS19-A,YP45,YS16A |
| 24 | X (V) | 40S ribosomal protein S20 | polymer | 121 | 13929.0 | 1 | UniProt (P38701) Pfam (PF00338) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Small ribosomal subunit protein uS10 |
| 25 | Y (W) | 40S ribosomal protein S21-A | polymer | 87 | 9758.8 | 1 | UniProt (P0C0V8) Pfam (PF01249) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | S26,Small ribosomal subunit protein eS21-A,YS25 |
| 26 | Z (X) | 40S ribosomal protein S22-A | polymer | 130 | 14650.1 | 1 | UniProt (P0C0W1) Pfam (PF00410) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP50,S24,Small ribosomal subunit protein uS8-A,YP58,YS22 |
| 27 | AA (Y) | 40S ribosomal protein S23-A | polymer | 145 | 16073.9 | 1 | UniProt (P0CX29) Pfam (PF00164) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP37,S28,Small ribosomal subunit protein uS12-A,YS14 |
| 28 | BA (Z) | 40S ribosomal protein S24-A | polymer | 135 | 15362.8 | 1 | UniProt (P0CX31) Pfam (PF01282) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP50,Small ribosomal subunit protein eS24-A |
| 29 | CA (a) | 40S ribosomal protein S25-A | polymer | 108 | 12067.3 | 1 | UniProt (Q3E792) Pfam (PF03297) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP45,S31,Small ribosomal subunit protein eS25-A,YS23 |
| 30 | DA (b) | 40S ribosomal protein S26-A | polymer | 119 | 13538.9 | 1 | UniProt (P39938) Pfam (PF01283) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Small ribosomal subunit protein eS26-A |
| 31 | EA (c) | 40S ribosomal protein S27-A | polymer | 82 | 8893.4 | 1 | UniProt (P35997) Pfam (PF01667) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RP61,Small ribosomal subunit protein eS27-A,YS20 |
| 32 | FA (d) | 40S ribosomal protein S28-B | polymer | 67 | 7578.8 | 1 | UniProt (P0C0X0) Pfam (PF01200) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | S33,Small ribosomal subunit protein eS28-B,YS27 |
| 33 | GA (e) | 40S ribosomal protein S29-A | polymer | 56 | 6675.7 | 1 | UniProt (P41057) Pfam (PF00253) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | S36,Small ribosomal subunit protein uS14-A,YS29 |
| 34 | HA (f) | 40S ribosomal protein S30-A | polymer | 63 | 7137.5 | 1 | UniProt (P0CX33) Pfam (PF04758) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Small ribosomal subunit protein eS30-A |
| 35 | IA (g) | Ubiquitin-40S ribosomal protein S31 | polymer | 152 | 17254.2 | 1 | UniProt (P05759) Pfam (PF00240) Pfam (PF01599) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 36 | JA (h) | Guanine nucleotide-binding protein subunit beta-like protein | polymer | 319 | 34841.2 | 1 | UniProt (P38011) Pfam (PF00400) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Receptor for activated C kinase,Receptor of activated protein kinase C 1,RACK1,Small ribosomal subunit protein RACK1 |
| 37 | KA (o) | Eukaryotic translation initiation factor 3 subunit A | polymer | 964 | 110517.6 | 1 | UniProt (P38249) Pfam (PF22591) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | eIF3a,Eukaryotic translation initiation factor 3 110 kDa subunit homolog,eIF3 p110,Translation initiation factor eIF3,p110 subunit homolog |
| 38 | LA (p) | Eukaryotic translation initiation factor 3 subunit B | polymer | 763 | 88241.8 | 1 | UniProt (P06103) Pfam (PF00076) Pfam (PF08662) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | eIF3b,Cell cycle regulation and translation initiation protein,Eukaryotic translation initiation factor 3 90 kDa subunit,eIF3 p90,Translation initiation factor eIF3 p90 subunit |
| 39 | MA (q) | Eukaryotic translation initiation factor 3 subunit C | polymer | 812 | 93310.2 | 1 | UniProt (P32497) Pfam (PF05470) Pfam (PF01399) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | eIF3c,Eukaryotic translation initiation factor 3 93 kDa subunit,eIF3 p93,Nuclear transport protein NIP1,Translation initiation factor eIF3,p93 subunit |
| 40 | NA (i) | Eukaryotic translation initiation factor 1A | polymer | 153 | 17462.2 | 1 | UniProt (P38912) Pfam (PF01176) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | eIF-1A,Eukaryotic translation initiation factor 4C,eIF-4C |
| 41 | OA (m) | Eukaryotic translation initiation factor eIF-1 | polymer | 108 | 12330.1 | 1 | UniProt (P32911) Pfam (PF01253) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Protein translation factor SUI1 |
| 42 | PA, SA (t, s) | Eukaryotic translation initiation factor 3 subunit J | polymer | 265 | 29585.2 | 2 | UniProt (Q05775) Pfam (PF08597) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | eIF3j,Eukaryotic translation initiation factor 3 30 kDa subunit,eIF-3 30 kDa |
| 43 | QA (j) | RNA recognition motif (unknown) | polymer | 77 | 5491.0 | 1 | Saccharomyces cerevisiae S288C | ||
| 44 | RA (k) | Translation initiation factor RLI1 | polymer | 608 | 68461.3 | 1 | UniProt (Q03195) Pfam (PF04068) Pfam (PF00037) Pfam (PF00005) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ATP-binding cassette sub-family E member RLI1,RNase L inhibitor |
| 45 | TA, UA, VA, WA (A, b, c, g) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 46 | XA (k) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 47 | AB, YA (k) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 48 | ZA (k) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 49 | BB, CB (k) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) |
Sequence modifications
r: 1 - 274 (UniProt: Q04067)
M: 2 - 156 (UniProt: P0CX47)
Q: 2 - 142 (UniProt: Q01855)
t, s: 1 - 265 (UniProt: Q05775)
k: 1 - 608 (UniProt: Q03195)
| PDB | External Database | Details |
|---|---|---|
| Ser 139 | Asn 139 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 147 | Gly 147 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 137 | Arg 137 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 254 | Pro 254 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Val 350 | Ala 350 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 45 |
| Total formula weight | 1698944.4 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1947.9 | |
| All* | Total formula weight | 1700892.3 |
