6Z6G
Cryo-EM structure of La Crosse virus polymerase at pre-initiation stage
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (U) | 5'vRNA 1-10 | polymer | 10 | 3218.0 | 1 | La Crosse orthobunyavirus | ||
| 2 | B (X) | 5'vRNA 9-16 | polymer | 8 | 2509.6 | 1 | La Crosse orthobunyavirus | ||
| 3 | C (H) | 3'vRNA 1-16 | polymer | 16 | 5060.0 | 1 | La Crosse orthobunyavirus | ||
| 4 | D (A) | RNA-directed RNA polymerase L | polymer | 2285 | 266047.5 | 1 | UniProt (A5HC98) Pfam (PF15518) Pfam (PF04196) Pfam (PF21561) | Bunyavirus La Crosse | Protein L,Large structural protein,Replicase,Transcriptase |
| 5 | E (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 1 - 2263 (UniProt: A5HC98)
| PDB | External Database | Details |
|---|---|---|
| Met -21 | - | initiating methionine |
| Gly -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Tyr -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Ile -10 | - | expression tag |
| Pro -9 | - | expression tag |
| Thr -8 | - | expression tag |
| Thr -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Asn -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 276835.0 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 89.7 | |
| All* | Total formula weight | 276924.7 |
