6Z3J
Repulsive Guidance Molecule B (RGMB) in complex with Growth Differentiation Factor 5 (GDF5) (crystal form 1)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B (A, B) | Growth/differentiation factor 5 | polymer | 117 | 13358.4 | 2 | UniProt (P43026) Pfam (PF00019) | Homo sapiens (Human) | GDF-5,Bone morphogenetic protein 14,BMP-14,Cartilage-derived morphogenetic protein 1,CDMP-1,Lipopolysaccharide-associated protein 4,LPS-associated protein 4,Radotermin |
2 | C, D (C, D) | RGM domain family member B | polymer | 96 | 10532.7 | 2 | UniProt (Q6NW40) Pfam (PF06535) | Homo sapiens (Human) | DRG11-responsive axonal guidance and outgrowth of neurite,DRAGON |
3 | E, J (A, C) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
4 | F, H (A, B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
5 | G, K, N (B, C, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
6 | I, L, O (B, C, D) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
7 | M (D) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
8 | P, Q, R, S (A, B, C, D) | water | water | 18.0 | 142 | Chemie (HOH) |
Sequence modifications
A, B: 387 - 501 (UniProt: P43026)
C, D: 53 - 136 (UniProt: Q6NW40)
PDB | External Database | Details |
---|---|---|
Met 385 | - | initiating methionine |
Lys 386 | - | expression tag |
Lys 487 | Tyr 487 | engineered mutation |
Asp 489 | Gln 489 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Glu 50 | - | expression tag |
Thr 51 | - | expression tag |
Gly 52 | - | expression tag |
Gly 137 | - | expression tag |
Thr 138 | - | expression tag |
Lys 139 | - | expression tag |
His 140 | - | expression tag |
His 141 | - | expression tag |
His 142 | - | expression tag |
His 143 | - | expression tag |
His 144 | - | expression tag |
His 145 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 47782.3 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 890.1 | |
All* | Total formula weight | 48672.4 |