6Z3J

Repulsive Guidance Molecule B (RGMB) in complex with Growth Differentiation Factor 5 (GDF5) (crystal form 1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Growth/differentiation factor 5	polymer	117	13358.4	2	UniProt (P43026) Pfam (PF00019)	Homo sapiens (Human)	GDF-5,Bone morphogenetic protein 14,BMP-14,Cartilage-derived morphogenetic protein 1,CDMP-1,Lipopolysaccharide-associated protein 4,LPS-associated protein 4,Radotermin
2	C, D (C, D)	RGM domain family member B	polymer	96	10532.7	2	UniProt (Q6NW40) Pfam (PF06535)	Homo sapiens (Human)	DRG11-responsive axonal guidance and outgrowth of neurite,DRAGON
3	E, J (A, C)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	F, H (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	G, K, N (B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
6	I, L, O (B, C, D)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
7	M (D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
8	P, Q, R, S (A, B, C, D)	water	water		18.0	142	Chemie (HOH)

Sequence modifications

A, B: 387 - 501 (UniProt: P43026)

PDB	External Database	Details
Met 385	-	initiating methionine
Lys 386	-	expression tag
Lys 487	Tyr 487	engineered mutation
Asp 489	Gln 489	engineered mutation

C, D: 53 - 136 (UniProt: Q6NW40)

PDB	External Database	Details
Glu 50	-	expression tag
Thr 51	-	expression tag
Gly 52	-	expression tag
Gly 137	-	expression tag
Thr 138	-	expression tag
Lys 139	-	expression tag
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	47782.3
Non-Polymers*	Number of molecules	11
	Total formula weight	890.1
All*	Total formula weight	48672.4

*Water molecules are not included.