6YXH

Cryogenic human alkaline ceramidase 3 (ACER3) at 2.6 A resolution determined by Serial Crystallography (SSX) using CrystalDirect

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Alkaline ceramidase 3	polymer	350	40491.1	1	UniProt (Q9NUN7) Pfam (PF05875)	Homo sapiens (Human)	Alkaline CDase 3,Alkaline dihydroceramidase SB89,Alkaline phytoceramidase,aPHC
2	B (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	C, D, E, F, G... (A)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
4	I, J (A)	SODIUM ION	non-polymer		23.0	2	Chemie (NA) PubChem (923)
5	K (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA) PubChem (271)
6	L, M (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
7	N (A)	water	water		18.0	60	Chemie (HOH)

Sequence modifications

A: 2 - 244 (UniProt: Q9NUN7)

PDB	External Database	Details
Phe 1	-	expression tag
Ala 245	-	expression tag
Asp 246	-	expression tag
Leu 247	-	expression tag
Glu 248	-	expression tag
Asp 249	-	expression tag
Asn 250	-	expression tag
Trp 251	-	expression tag
Glu 252	-	expression tag
Thr 253	-	expression tag
Leu 254	-	expression tag
Asn 255	-	expression tag
Asp 256	-	expression tag
Asn 257	-	expression tag
Leu 258	-	expression tag
Lys 259	-	expression tag
Val 260	-	expression tag
Ile 261	-	expression tag
Glu 262	-	expression tag
Lys 263	-	expression tag
Ala 264	-	expression tag
Asp 265	-	expression tag
Asn 266	-	expression tag
Ala 267	-	expression tag
Ala 268	-	expression tag
Gln 269	-	expression tag
Val 270	-	expression tag
Lys 271	-	expression tag
Asp 272	-	expression tag
Ala 273	-	expression tag
Leu 274	-	expression tag
Thr 275	-	expression tag
Lys 276	-	expression tag
Met 277	-	expression tag
Arg 278	-	expression tag
Ala 279	-	expression tag
Ala 280	-	expression tag
Ala 281	-	expression tag
Leu 282	-	expression tag
Asp 283	-	expression tag
Ala 284	-	expression tag
Gln 285	-	expression tag
Lys 286	-	expression tag
Ala 287	-	expression tag
Thr 288	-	expression tag
Pro 289	-	expression tag
Pro 290	-	expression tag
Lys 291	-	expression tag
Leu 292	-	expression tag
Glu 293	-	expression tag
Asp 294	-	expression tag
Lys 295	-	expression tag
Ser 296	-	expression tag
Pro 297	-	expression tag
Asp 298	-	expression tag
Ser 299	-	expression tag
Pro 300	-	expression tag
Glu 301	-	expression tag
Met 302	-	expression tag
Lys 303	-	expression tag
Asp 304	-	expression tag
Phe 305	-	expression tag
Arg 306	-	expression tag
His 307	-	expression tag
Gly 308	-	expression tag
Phe 309	-	expression tag
Asp 310	-	expression tag
Ile 311	-	expression tag
Leu 312	-	expression tag
Val 313	-	expression tag
Gly 314	-	expression tag
Gln 315	-	expression tag
Ile 316	-	expression tag
Asp 317	-	expression tag
Asp 318	-	expression tag
Ala 319	-	expression tag
Leu 320	-	expression tag
Lys 321	-	expression tag
Leu 322	-	expression tag
Ala 323	-	expression tag
Asn 324	-	expression tag
Glu 325	-	expression tag
Gly 326	-	expression tag
Lys 327	-	expression tag
Val 328	-	expression tag
Lys 329	-	expression tag
Glu 330	-	expression tag
Ala 331	-	expression tag
Gln 332	-	expression tag
Ala 333	-	expression tag
Ala 334	-	expression tag
Ala 335	-	expression tag
Glu 336	-	expression tag
Gln 337	-	expression tag
Leu 338	-	expression tag
Lys 339	-	expression tag
Thr 340	-	expression tag
Thr 341	-	expression tag
Arg 342	-	expression tag
Asn 343	-	expression tag
Ala 344	-	expression tag
Tyr 345	-	expression tag
Ile 346	-	expression tag
Gln 347	-	expression tag
Lys 348	-	expression tag
Tyr 349	-	expression tag
Leu 350	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40491.1
Non-Polymers*	Number of molecules	12
	Total formula weight	776.5
All*	Total formula weight	41267.5

*Water molecules are not included.