6YS3
Cryo-EM structure of the 50S ribosomal subunit at 2.58 Angstroms with modeled GBC SecM peptide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (0) | 50S ribosomal protein L28 | polymer | 78 | 9027.6 | 1 | UniProt (A0A140N3L9) Pfam (PF00830) UniProt (by SIFTS) (P0A7M2) | Escherichia coli BL21(DE3) | |
| 2 | B (1) | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (A0A140N7F5) Pfam (PF00831) UniProt (by SIFTS) (P0A7M6) | Escherichia coli BL21(DE3) | |
| 3 | C (2) | 50S ribosomal protein L30 | polymer | 59 | 6554.8 | 1 | UniProt (A0A140N7G4) Pfam (PF00327) UniProt (by SIFTS) (P0AG51) | Escherichia coli BL21(DE3) | |
| 4 | D (3) | 50S ribosomal protein L32 | polymer | 57 | 6463.4 | 1 | UniProt (A0A140NAZ2) Pfam (PF01783) UniProt (by SIFTS) (P0A7N4) | Escherichia coli BL21(DE3) | |
| 5 | E (4) | 50S ribosomal protein L33 | polymer | 55 | 6388.6 | 1 | UniProt (A0A140N5Z7) Pfam (PF00471) UniProt (by SIFTS) (P0A7N9) | Escherichia coli BL21(DE3) | |
| 6 | F (6) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (A0A140NHV0) Pfam (PF00468) UniProt (by SIFTS) (P0A7P5) | Escherichia coli BL21(DE3) | |
| 7 | G (7) | 50S ribosomal protein L35 | polymer | 65 | 7313.0 | 1 | UniProt (A0A140N9E3) Pfam (PF01632) UniProt (by SIFTS) (P0A7Q1) | Escherichia coli BL21(DE3) | |
| 8 | H (8) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (A0A140N5M6) Pfam (PF00444) UniProt (by SIFTS) (P0A7Q6) | Escherichia coli BL21(DE3) | |
| 9 | I (a) | 5S rRNA | polymer | 120 | 38790.1 | 1 | Escherichia coli BL21(DE3) | ||
| 10 | J (b) | 23S rRNA | polymer | 2906 | 942342.9 | 1 | GenBank (296142109) | Escherichia coli BL21(DE3) | |
| 11 | K (c) | 50S ribosomal protein L2 | polymer | 273 | 29923.6 | 1 | UniProt (A0A140N7J1) Pfam (PF03947) Pfam (PF00181) UniProt (by SIFTS) (P60422) | Escherichia coli BL21(DE3) | |
| 12 | L (d) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (A0A140N3G7) Pfam (PF00297) UniProt (by SIFTS) (P60438) | Escherichia coli BL21(DE3) | |
| 13 | M (e) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (A0A140N5K8) Pfam (PF00573) UniProt (by SIFTS) (P60723) | Escherichia coli BL21(DE3) | |
| 14 | N (f) | 50S ribosomal protein L5 | polymer | 179 | 20333.6 | 1 | UniProt (A0A140N5A3) Pfam (PF00281) Pfam (PF00673) UniProt (by SIFTS) (P62399) | Escherichia coli BL21(DE3) | |
| 15 | O (g) | 50S ribosomal protein L6 | polymer | 177 | 18932.8 | 1 | UniProt (A0A140N2T1) Pfam (PF00347) UniProt (by SIFTS) (P0AG55) | Escherichia coli BL21(DE3) | |
| 16 | P (h) | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (A0A140NDV1) Pfam (PF01281) Pfam (PF03948) UniProt (by SIFTS) (P0A7R1) | Escherichia coli BL21(DE3) | |
| 17 | Q (j) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (A0A140N598) Pfam (PF00572) UniProt (by SIFTS) (P0AA10) | Escherichia coli BL21(DE3) | |
| 18 | R (k) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (A0A140N3H4) Pfam (PF00238) UniProt (by SIFTS) (P0ADY3) | Escherichia coli BL21(DE3) | |
| 19 | S (l) | 50S ribosomal protein L15 | polymer | 144 | 14994.4 | 1 | UniProt (A0A140N711) Pfam (PF00828) UniProt (by SIFTS) (P02413) | Escherichia coli BL21(DE3) | |
| 20 | T (m) | 50S ribosomal protein L16 | polymer | 136 | 15312.3 | 1 | UniProt (A0A140N6Z2) Pfam (PF00252) UniProt (by SIFTS) (P0ADY7) | Escherichia coli BL21(DE3) | |
| 21 | U (n) | 50S ribosomal protein L17 | polymer | 127 | 14393.7 | 1 | UniProt (A0A140N4M0) Pfam (PF01196) UniProt (by SIFTS) (P0AG44) | Escherichia coli BL21(DE3) | |
| 22 | V (o) | 50S ribosomal protein L18 | polymer | 117 | 12794.7 | 1 | UniProt (A0A140N4L0) UniProt (by SIFTS) (P0ADY7) | Escherichia coli BL21(DE3) | |
| 23 | W (p) | 50S ribosomal protein L19 | polymer | 115 | 13159.3 | 1 | UniProt (A0A140N6T7) Pfam (PF01245) UniProt (by SIFTS) (P0A7K6) | Escherichia coli BL21(DE3) | |
| 24 | X (q) | 50S ribosomal protein L20 | polymer | 118 | 13528.0 | 1 | UniProt (A0A140NBS1) Pfam (PF00453) UniProt (by SIFTS) (P0A7L3) | Escherichia coli BL21(DE3) | |
| 25 | Y (r) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (A0A140N5D7) Pfam (PF00829) UniProt (by SIFTS) (P0AG48) | Escherichia coli BL21(DE3) | |
| 26 | Z (s) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (A0A140N2S3) Pfam (PF00237) UniProt (by SIFTS) (P61175) | Escherichia coli BL21(DE3) | |
| 27 | AA (t) | 50S ribosomal protein L23 | polymer | 100 | 11222.2 | 1 | UniProt (C6EGE8) Pfam (PF00276) UniProt (by SIFTS) (P0ADZ0) | Escherichia coli BL21(DE3) | |
| 28 | BA (u) | 50S ribosomal protein L24 | polymer | 104 | 11339.3 | 1 | UniProt (A0A140N5L7) Pfam (PF00467) Pfam (PF17136) UniProt (by SIFTS) (P60624) | Escherichia coli BL21(DE3) | |
| 29 | CA (v) | glycine-tRNA glyT | polymer | 75 | 23935.1 | 1 | Escherichia coli BL21(DE3) | ||
| 30 | DA (w) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (A0A140N846) Pfam (PF01386) UniProt (by SIFTS) (P68919) | Escherichia coli BL21(DE3) | |
| 31 | EA (y) | 50S ribosomal protein L27 | polymer | 85 | 9146.5 | 1 | UniProt (A0A140N340) Pfam (PF01016) UniProt (by SIFTS) (P0A7L8) | Escherichia coli BL21(DE3) | |
| 32 | FA (z) | Gamma-crystallin B | polymer | 61 | 7158.0 | 1 | UniProt (P02526) Pfam (PF00030) | Bos taurus (Cattle) | Gamma-B-crystallin,Gamma-crystallin II |
Sequence modifications
z: 0 - 154 (UniProt: P02526)
| PDB | External Database | Details |
|---|---|---|
| Met -12 | - | initiating methionine |
| Gly -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| Phe 150 | Asn 34 | conflict |
| Thr 152 | Ile 36 | conflict |
| Pro 153 | Arg 37 | conflict |
| Trp 155 | - | expression tag |
| Ile 156 | - | expression tag |
| Ser 157 | - | expression tag |
| Gln 158 | - | expression tag |
| Ala 159 | - | expression tag |
| Gln 160 | - | expression tag |
| Gly 161 | - | expression tag |
| Ile 162 | - | expression tag |
| Arg 163 | - | expression tag |
| Ala 164 | - | expression tag |
| Gly 165 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 32 |
| Total formula weight | 1374472.2 | |
| All* | Total formula weight | 1374472.2 |
