6YPA

The C146A variant of an amidase from Pyrococcus horikoshii with bound glutaramide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	CN hydrolase domain-containing protein	polymer	282	31983.7	4	UniProt (O58376) Pfam (PF00795) In PDB	Pyrococcus horikoshii
2	A, B, C, D	pentanediamide	non-polymer		130.1	4	Chemie (P6W)
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4		water	water		18.0	556	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 262 (UniProt: O58376)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 146	Cys 146	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	127934.8
Non-Polymers*	Number of molecules	5
	Total formula weight	612.7
All*	Total formula weight	128547.5

*Water molecules are not included.