6YMW
Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Mitochondrial transcription factor 1 | polymer | 354 | 41151.2 | 1 | UniProt (P14908) Pfam (PF00398) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Mitochondrial transcription factor mtTFB,Mitochondrial-specificity factor,RF1023 |
| 2 | B (A) | DNA-directed RNA polymerase, mitochondrial | polymer | 1262 | 143282.7 | 1 | UniProt (P13433) Pfam (PF14700) Pfam (PF00940) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 3 | C (N) | Chains: N | polymer | 33 | 10248.7 | 1 | Saccharomyces cerevisiae S288C | ||
| 4 | D (T) | Chains: T | polymer | 33 | 10047.5 | 1 | Saccharomyces cerevisiae S288C | ||
| 5 | E (C) | RNA (pppGpG) | polymer | 2 | 805.4 | 1 | synthetic construct | ||
| 6 | F (A) | [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl] phosphono hydrogen phosphate | non-polymer | 500.2 | 1 | Chemie (P5E) | |||
| 7 | G (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
B: 2 - 341 (UniProt: P14908)
A: 100 - 1351 (UniProt: P13433)
| PDB | External Database | Details |
|---|---|---|
| Met -12 | - | initiating methionine |
| Gly -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Ser -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ser 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 90 | - | expression tag |
| Ala 91 | - | expression tag |
| Met 92 | - | expression tag |
| Gly 93 | - | expression tag |
| Ser 94 | - | expression tag |
| Gly 95 | - | expression tag |
| Ile 96 | - | expression tag |
| Gln 97 | - | expression tag |
| Arg 98 | - | expression tag |
| Pro 99 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 205535.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 524.5 | |
| All* | Total formula weight | 206060.0 |
