6YMH
X-ray structure of the K72I, Y129F, R133L, H199A quadruple mutant of PNP-oxidase from E. coli in complex with PLP
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (AAA, BBB) | Pyridoxine/pyridoxamine 5'-phosphate oxidase | polymer | 218 | 25442.1 | 2 | UniProt (P0AFI7) Pfam (PF01243) Pfam (PF10590) | Escherichia coli (strain K12) | PNP/PMP oxidase,PNPOx,Pyridoxal 5'-phosphate synthase |
| 2 | C, E (AAA, BBB) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 2 | Chemie (FMN) | |||
| 3 | D (AAA) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 4 | F (BBB) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 1 | Chemie (PLP) | |||
| 5 | G, H (AAA, BBB) | water | water | 18.0 | 45 | Chemie (HOH) |
Sequence modifications
AAA, BBB: 1 - 218 (UniProt: P0AFI7)
| PDB | External Database | Details |
|---|---|---|
| Ile 72 | Lys 72 | engineered mutation |
| Phe 129 | Tyr 129 | engineered mutation |
| Leu 133 | Arg 133 | engineered mutation |
| Ala 199 | His 199 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 50884.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1255.9 | |
| All* | Total formula weight | 52140.0 |
