6XOX

cryo-EM of human GLP-1R bound to non-peptide agonist LY3502970

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Alpha subunit of Gs with N-terminus swapped with equivalent residues in Gi,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas	polymer	373	43385.2	1	UniProt (Q5FWY2) UniProt (Q5JWF2) Pfam (PF00503) UniProt (by SIFTS) (P63092)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein,Extra large alphas protein,XLalphas
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (E)	single-chain variable fragment scFv16	polymer	259	27784.9	1		Mus musculus (mouse)
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
5	E (N)	Nanobody 35	polymer	160	17352.5	1		Lama glama
6	F (R)	Glucagon-like peptide 1 receptor	polymer	445	51233.1	1	UniProt (P43220) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (Human)	GLP-1R
7	G (R)	3-[(1S,2S)-1-(5-[(4S)-2,2-dimethyloxan-4-yl]-2-{(4S)-2-(4-fluoro-3,5-dimethylphenyl)-3-[3-(4-fluoro-1-methyl-1H-indazol-5-yl)-2-oxo-2,3-dihydro-1H-imidazol-1-yl]-4-methyl-2,4,6,7-tetrahydro-5H-pyrazolo[4,3-c]pyridine-5-carbonyl}-1H-indol-1-yl)-2-methylcyclopropyl]-1,2,4-oxadiazol-5(4H)-one	non-polymer		883.0	1	Chemie (V6G)

Sequence modifications

A: 26 - 86 (UniProt: Q5FWY2)

PDB	External Database	Details
Met 8	-	initiating methionine
Gly 9	-	expression tag
Cys 10	-	expression tag
Thr 11	-	expression tag
Leu 12	-	expression tag
Ser 13	-	expression tag
Ala 14	-	expression tag
Glu 15	-	expression tag
Asp 16	-	expression tag
Lys 17	-	expression tag
Ala 18	-	expression tag
Ala 19	-	expression tag
Val 20	-	expression tag
Glu 21	-	expression tag
Arg 22	-	expression tag
Ser 23	-	expression tag
Lys 24	-	expression tag
Met 25	-	expression tag

A: 87 - 394 (UniProt: Q5JWF2)
B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 24 - 422 (UniProt: P43220)

PDB	External Database	Details
Met -22	-	initiating methionine
Lys -21	-	expression tag
Thr -20	-	expression tag
Ile -19	-	expression tag
Ile -18	-	expression tag
Ala -17	-	expression tag
Leu -16	-	expression tag
Ser -15	-	expression tag
Tyr -14	-	expression tag
Ile -13	-	expression tag
Phe -12	-	expression tag
Cys -11	-	expression tag
Leu -10	-	expression tag
Val -9	-	expression tag
Phe -8	-	expression tag
Ala -7	-	expression tag
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Ala 1	-	expression tag
Ala 2	-	expression tag
Ala 3	-	expression tag
Gly 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag
Gly 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Leu 10	-	expression tag
Glu 11	-	expression tag
Val 12	-	expression tag
Leu 13	-	expression tag
Phe 14	-	expression tag
Gln 15	-	expression tag
Gly 16	-	expression tag
Pro 17	-	expression tag
Gly 18	-	expression tag
Gly 19	-	expression tag
Ser 20	-	expression tag
Gly 21	-	expression tag
Gly 22	-	expression tag
Ser 23	-	expression tag
Phe 260	Leu 260	variant

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	186151.0
Non-Polymers*	Number of molecules	1
	Total formula weight	883.0
All*	Total formula weight	187033.9

*Water molecules are not included.