6XOX
cryo-EM of human GLP-1R bound to non-peptide agonist LY3502970
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alpha subunit of Gs with N-terminus swapped with equivalent residues in Gi,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas | polymer | 373 | 43385.2 | 1 | UniProt (Q5FWY2) UniProt (Q5JWF2) Pfam (PF00503) UniProt (by SIFTS) (P63092) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein,Extra large alphas protein,XLalphas |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 350 | 38534.1 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C (E) | single-chain variable fragment scFv16 | polymer | 259 | 27784.9 | 1 | Mus musculus (mouse) | ||
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 5 | E (N) | Nanobody 35 | polymer | 160 | 17352.5 | 1 | Lama glama | ||
| 6 | F (R) | Glucagon-like peptide 1 receptor | polymer | 445 | 51233.1 | 1 | UniProt (P43220) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (Human) | GLP-1R |
| 7 | G (R) | 3-[(1S,2S)-1-(5-[(4S)-2,2-dimethyloxan-4-yl]-2-{(4S)-2-(4-fluoro-3,5-dimethylphenyl)-3-[3-(4-fluoro-1-methyl-1H-indazol-5-yl)-2-oxo-2,3-dihydro-1H-imidazol-1-yl]-4-methyl-2,4,6,7-tetrahydro-5H-pyrazolo[4,3-c]pyridine-5-carbonyl}-1H-indol-1-yl)-2-methylcyclopropyl]-1,2,4-oxadiazol-5(4H)-one | non-polymer | 883.0 | 1 | Chemie (V6G) PubChem (176488769) PubChem (176488787) PubChem (176488794) PubChem (176488796) PubChem (176488798) PubChem (176488800) PubChem (176488801) PubChem (176488802) PubChem (176488803) PubChem (176488760) PubChem (176488785) PubChem (176488799) PubChem (176488804) PubChem (137319706) PubChem (155369847) PubChem (155369852) |
Sequence modifications
A: 26 - 86 (UniProt: Q5FWY2)
A: 87 - 394 (UniProt: Q5JWF2)
B: 2 - 340 (UniProt: P62873)
R: 24 - 422 (UniProt: P43220)
| PDB | External Database | Details |
|---|---|---|
| Met 8 | - | initiating methionine |
| Gly 9 | - | expression tag |
| Cys 10 | - | expression tag |
| Thr 11 | - | expression tag |
| Leu 12 | - | expression tag |
| Ser 13 | - | expression tag |
| Ala 14 | - | expression tag |
| Glu 15 | - | expression tag |
| Asp 16 | - | expression tag |
| Lys 17 | - | expression tag |
| Ala 18 | - | expression tag |
| Ala 19 | - | expression tag |
| Val 20 | - | expression tag |
| Glu 21 | - | expression tag |
| Arg 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Lys 24 | - | expression tag |
| Met 25 | - | expression tag |
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Met -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -22 | - | initiating methionine |
| Lys -21 | - | expression tag |
| Thr -20 | - | expression tag |
| Ile -19 | - | expression tag |
| Ile -18 | - | expression tag |
| Ala -17 | - | expression tag |
| Leu -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Tyr -14 | - | expression tag |
| Ile -13 | - | expression tag |
| Phe -12 | - | expression tag |
| Cys -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Val -9 | - | expression tag |
| Phe -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Ala 1 | - | expression tag |
| Ala 2 | - | expression tag |
| Ala 3 | - | expression tag |
| Gly 4 | - | expression tag |
| Gly 5 | - | expression tag |
| Ser 6 | - | expression tag |
| Gly 7 | - | expression tag |
| Gly 8 | - | expression tag |
| Ser 9 | - | expression tag |
| Leu 10 | - | expression tag |
| Glu 11 | - | expression tag |
| Val 12 | - | expression tag |
| Leu 13 | - | expression tag |
| Phe 14 | - | expression tag |
| Gln 15 | - | expression tag |
| Gly 16 | - | expression tag |
| Pro 17 | - | expression tag |
| Gly 18 | - | expression tag |
| Gly 19 | - | expression tag |
| Ser 20 | - | expression tag |
| Gly 21 | - | expression tag |
| Gly 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Phe 260 | Leu 260 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 186151.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 883.0 | |
| All* | Total formula weight | 187033.9 |
