6XNX
Structure of RAG1 (R848M/E649V)-RAG2-DNA Strand Transfer Complex (Dynamic-Form)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | V(D)J recombination-activating protein 1 | polymer | 750 | 85750.8 | 2 | UniProt (P15919) Pfam (PF00097) Pfam (PF10426) Pfam (PF12940) | Mus musculus (Mouse) | RAG-1 |
| 2 | B, D (B, D) | V(D)J recombination-activating protein 2 | polymer | 363 | 40417.0 | 2 | UniProt (P21784) Pfam (PF03089) | Mus musculus (Mouse) | RAG-2 |
| 3 | E (x) | 12RSS integration strand DNA (55-MER) | polymer | 55 | 16901.8 | 1 | Homo sapiens | ||
| 4 | F (y) | 23RSS integration strand DNA (66-MER) | polymer | 66 | 20323.0 | 1 | Homo sapiens | ||
| 5 | G, H (I, J) | Flanking DNA top strand DNA | polymer | 16 | 4923.2 | 2 | Homo sapiens | ||
| 6 | I (L) | 23RSS signal top strand DNA (45-MER) | polymer | 45 | 13877.9 | 1 | Homo sapiens | ||
| 7 | J (M) | 12RSS signal top strand DNA (34-MER) | polymer | 34 | 10461.8 | 1 | Homo sapiens | ||
| 8 | K, L, N, P (A, C, x) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 9 | M, O (A, C) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, C: 261 - 1008 (UniProt: P15919)
B, D: 3 - 361 (UniProt: P21784)
| PDB | External Database | Details |
|---|---|---|
| Gly 259 | - | expression tag |
| Pro 260 | - | expression tag |
| Val 649 | Glu 649 | engineered mutation |
| Met 848 | Arg 848 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Met 1 | - | expression tag |
| Ala 2 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 323746.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 228.0 | |
| All* | Total formula weight | 323974.4 |
