6XNX

Structure of RAG1 (R848M/E649V)-RAG2-DNA Strand Transfer Complex (Dynamic-Form)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004519	molecular_function	endonuclease activity
A	0033151	biological_process	V(D)J recombination
A	0043565	molecular_function	sequence-specific DNA binding
A	0046872	molecular_function	metal ion binding
A	0061630	molecular_function	ubiquitin protein ligase activity
B	0003677	molecular_function	DNA binding
B	0005634	cellular_component	nucleus
B	0006310	biological_process	DNA recombination
C	0004519	molecular_function	endonuclease activity
C	0033151	biological_process	V(D)J recombination
C	0043565	molecular_function	sequence-specific DNA binding
C	0046872	molecular_function	metal ion binding
C	0061630	molecular_function	ubiquitin protein ligase activity
D	0003677	molecular_function	DNA binding
D	0005634	cellular_component	nucleus
D	0006310	biological_process	DNA recombination

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue MG A 1101

Chain	Residue
A	GLY601
A	MET602
y	DG35
y	DC36

---

site_id	AC2
Number of Residues	3
Details	binding site for residue MG A 1102

Chain	Residue
A	GLU662
A	ASP708
I	DC16

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN A 1103

Chain	Residue
A	HIS937
A	HIS942
A	CYS727
A	CYS730

---

site_id	AC4
Number of Residues	6
Details	binding site for residue MG C 1101

Chain	Residue
C	GLY601
C	GLU662
C	ASP708
J	DC16
x	DC47
x	MG101

---

site_id	AC5
Number of Residues	4
Details	binding site for residue ZN C 1102

Chain	Residue
C	CYS727
C	CYS730
C	HIS937
C	HIS942

---

site_id	AC6
Number of Residues	4
Details	binding site for residue MG x 101

Chain	Residue
C	GLY601
C	MG1101
x	DG46
x	DC47

---

Functional Information from PROSITE/UniProt

site_id	PS00435
Number of Residues	11
Details	PEROXIDASE_1 Peroxidases proximal heme-ligand signature. DTVYILGGHSL

Chain	Residue	Details
B	ASP214-LEU224

---

site_id	PS00518
Number of Residues	10
Details	ZF_RING_1 Zinc finger RING-type signature. CkHlFCriCI

Chain	Residue	Details
A	CYS305-ILE314

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	78
Details	ZN_FING: RING-type => ECO:0000255|PROSITE-ProRule:PRU00175

Chain	Residue	Details
A	CYS290-ARG329
C	CYS290-ARG329

---

site_id	SWS_FT_FI2
Number of Residues	58
Details	ZN_FING: RAG1-type => ECO:0000255|PROSITE-ProRule:PRU01101

Chain	Residue	Details
A	LEU351-LYS380
C	LEU351-LYS380

---

site_id	SWS_FT_FI3
Number of Residues	134
Details	DNA_BIND: NBD => ECO:0000255|PROSITE-ProRule:PRU00820

Chain	Residue	Details
A	GLY389-GLN456
C	GLY389-GLN456

---

site_id	SWS_FT_FI4
Number of Residues	22
Details	BINDING: BINDING => ECO:0000269|PubMed:9228952

Chain	Residue	Details
A	CYS266
A	CYS325
A	CYS328
C	CYS266
C	HIS270
C	CYS290
C	CYS293
C	HIS295
C	CYS305
C	HIS307
C	CYS310
A	HIS270
C	CYS313
C	CYS325
C	CYS328
A	CYS290
A	CYS293
A	HIS295
A	CYS305
A	HIS307
A	CYS310
A	CYS313

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01101, ECO:0000269|PubMed:9228952

Chain	Residue	Details
A	CYS355
A	CYS360
A	HIS372
A	HIS376
C	CYS355
C	CYS360
C	HIS372
C	HIS376

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	BINDING: BINDING => ECO:0000305

Chain	Residue	Details
A	ASP600
A	ASP708
A	GLU962
C	ASP600
C	ASP708
C	GLU962

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	SITE: Essential for DNA hairpin formation, participates in base-stacking interactions near the cleavage site

Chain	Residue	Details
A	TRP893
C	TRP893

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